About Enhancer
| Enhancer ID: | E_01_0453 |
| Species: | human |
| Position : | chr8:19899275-19901275   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Nothing |
| Pubmed ID: | 29794473 |
| Enhancer experiment: | Double Dual Luciferase Reporter Assay,qRT-PCR,western blot,cellular immunofluorescence,oil red O staining assay,Cell transfection |
| Enhancer experiment description: | During the adipogenic differentiation of hASCs, miR-27b expression decreased, and LPL expression increased. The abnormal expression of miR-27b and LPL effectively regulated the adipogenic differentiation of hASCs. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | During the adipogenic differentiation of hASCs, miR-27b expression decreased, and LPL expression increased. The abnormal expression of miR-27b and LPL effectively regulated the adipogenic differentiation of hASCs. |
About TF
| TF name : | LPL |
| TF experiment: | Double Dual Luciferase Reporter Assay,qRT-PCR,western blot,cellular immunofluorescence,oil red O staining assay,Cell transfection |
| TF experiment description: | During the adipogenic differentiation of hASCs, miR-27b expression decreased, and LPL expression increased. The abnormal expression of miR-27b and LPL effectively regulated the adipogenic differentiation of hASCs. |
About Function
| Enhancer function : | During the adipogenic differentiation of hASCs, miR-27b expression decreased, and LPL expression increased. The abnormal expression of miR-27b and LPL effectively regulated the adipogenic differentiation of hASCs. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
During the adipogenic differentiation of hASCs, miR-27b expression decreased, and LPL expression increased. The abnormal expression of miR-27b and LPL effectively regulated the adipogenic differentiation of hASCs. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| LPL | Chylomicron-mediated lipid transport | reactome | 17 |
| LPL | D-glyceric acidura | smpdb | 11 |
| LPL | Familial lipoprotein lipase deficiency | smpdb | 11 |
| LPL | FOXA2 and FOXA3 transcription factor networks | pid | 45 |
| LPL | Glycerol Kinase Deficiency | smpdb | 11 |
| LPL | Glycerolipid Metabolism | smpdb | 11 |
| LPL | Retinoid metabolism and transport | reactome | 42 |
| LPL | Transcriptional regulation of white adipocyte differentiation | reactome | 79 |
| LPL | Glycerolipid metabolism | kegg | 49 |
| LPL | PPAR signaling pathway | kegg | 67 |
| LPL | Alzheimer's disease | kegg | 167 |
| LPL | Hs_Angiopoietin_Like_Protein_8_Regulatory_Pathway_WP3915_90629 | wikipathways | 9 |
| LPL | Hs_Composition_of_Lipid_Particles_WP3601_89202 | wikipathways | 5 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene