About Enhancer
| Enhancer ID: | E_01_0422 |
| Species: | human |
| Position : | chr13:110709998-110711998   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Premature ageing |
| Pubmed ID: | 29884649 |
| Enhancer experiment: | WGBS-seq,Oil Red O staining,RNA isolation,cDNA synthesis,qPCR,RNA-seq,RNA expression microarrays,ChIP,ChIP-seq,NG Capture-C,Methylation-sensitive PCR,Hematoxylin and eosin (H&E) staining,SA-?-Gal staining,Luciferase reporter assays,Co-IP,immunoblotting,Immunostaining |
| Enhancer experiment description: | Correspondingly, GADD45? occupies C/EBP?/?-dependent superenhancers and, cooperatively with ING1, promotes local DNA demethylation via long-range chromatin loops to permit C/EBP? recruitment. The results indicate that enhancer methylation can affect aging and imply that C/EBP proteins play an unexpected role in this process. Our study suggests a causal nexus between DNA demethylation, metabolism, and organismal aging. |
About Target gene
| Target gene : | ING1 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Correspondingly, GADD45? occupies C/EBP?/?-dependent superenhancers and, cooperatively with ING1, promotes local DNA demethylation via long-range chromatin loops to permit C/EBP? recruitment. The results indicate that enhancer methylation can affect aging and imply that C/EBP proteins play an unexpected role in this process. Our study suggests a causal nexus between DNA demethylation, metabolism, and organismal aging. |
About TF
| TF name : | -- |
| TF experiment: | WGBS-seq,Oil Red O staining,RNA isolation,cDNA synthesis,qPCR,RNA-seq,RNA expression microarrays,ChIP,ChIP-seq,NG Capture-C,Methylation-sensitive PCR,Hematoxylin and eosin (H&E) staining,SA-?-Gal staining,Luciferase reporter assays,Co-IP,immunoblotting,Immunostaining |
| TF experiment description: | Correspondingly, GADD45? occupies C/EBP?/?-dependent superenhancers and, cooperatively with ING1, promotes local DNA demethylation via long-range chromatin loops to permit C/EBP? recruitment. The results indicate that enhancer methylation can affect aging and imply that C/EBP proteins play an unexpected role in this process. Our study suggests a causal nexus between DNA demethylation, metabolism, and organismal aging. |
About Function
| Enhancer function : | Correspondingly, GADD45? occupies C/EBP?/?-dependent superenhancers and, cooperatively with ING1, promotes local DNA demethylation via long-range chromatin loops to permit C/EBP? recruitment. The results indicate that enhancer methylation can affect aging and imply that C/EBP proteins play an unexpected role in this process. Our study suggests a causal nexus between DNA demethylation, metabolism, and organismal aging. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Correspondingly, GADD45? occupies C/EBP?/?-dependent superenhancers and, cooperatively with ING1, promotes local DNA demethylation via long-range chromatin loops to permit C/EBP? recruitment. The results indicate that enhancer methylation can affect aging and imply that C/EBP proteins play an unexpected role in this process. Our study suggests a causal nexus between DNA demethylation, metabolism, and organismal aging. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene