About Enhancer

Enhancer ID: E_01_0283
Species: human
Position : chr6:151653980-151655980
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Mammary cancer
Pubmed ID:  31504407
Enhancer experiment: PCR, Western blot, immunofluorescence staining
Enhancer experiment description: We observed ESR1 mRNA and ER protein down_x0002_regulation in BC cells treated with conditioned media (CM) from patient-derived, cancer-activated bone marrow stromal cells (BMSCs) and the BMSC cell line HS5.

About Target gene

Target gene : --
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: We observed ESR1 mRNA and ER protein down_x0002_regulation in BC cells treated with conditioned media (CM) from patient-derived, cancer-activated bone marrow stromal cells (BMSCs) and the BMSC cell line HS5.

About TF

TF name : ESR1(ER,ESR,ESRA,ESTRR,Era,NR3A1)
TF experiment: PCR,Western blot,??????
TF experiment description: We observed ESR1 mRNA and ER protein down_x0002_regulation in BC cells treated with conditioned media (CM) from patient-derived, cancer-activated bone marrow stromal cells (BMSCs) and the BMSC cell line HS5.

About Function

Enhancer function : We observed ESR1 mRNA and ER protein down_x0002_regulation in BC cells treated with conditioned media (CM) from patient-derived, cancer-activated bone marrow stromal cells (BMSCs) and the BMSC cell line HS5.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 We observed ESR1 mRNA and ER protein down_x0002_regulation in BC cells treated with conditioned media (CM) from patient-derived, cancer-activated bone marrow stromal cells (BMSCs) and the BMSC cell line HS5.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
ESR1 AndrogenReceptor netpath 167
ESR1 AP-1 transcription factor network pid 71
ESR1 ATF-2 transcription factor network pid 59
ESR1 Cellular roles of Anthrax toxin pid 25
ESR1 FOXA1 transcription factor network pid 45
ESR1 FOXM1 transcription factor network pid 43
ESR1 Leptin netpath 98
ESR1 LKB1 signaling events pid 43
ESR1 Nuclear Receptor transcription pathway reactome 51
ESR1 Nuclear signaling by ERBB4 reactome 25
ESR1 Plasma membrane estrogen receptor signaling pid 42
ESR1 Prolactin netpath 105
ESR1 Regulation of nuclear SMAD2/3 signaling pid 82
ESR1 Regulation of Telomerase pid 70
ESR1 Signaling events mediated by HDAC Class II pid 58
ESR1 Signaling mediated by p38-alpha and p38-beta pid 35
ESR1 Tamoxifen Action Pathway smpdb 10
ESR1 Tamoxifen Metabolism Pathway smpdb 10
ESR1 TGF_beta_Receptor netpath 220
ESR1 Validated nuclear estrogen receptor alpha network pid 65
ESR1 Hs_DNA_Damage_Response_(only_ATM_dependent)_WP710_79974 wikipathways 36
ESR1 Hs_Leptin_signaling_pathway_WP2034_89856 wikipathways 37
ESR1 Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
ESR1 Hs_Aryl_Hydrocarbon_Receptor_WP2586_91687 wikipathways 16
ESR1 Hs_Estrogen_signaling_pathway_WP712_78491 wikipathways 13

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs