About Enhancer
| Enhancer ID: | E_01_0233 |
| Species: | human |
| Position : | chr12:6531857-6533857   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Obesity |
| Pubmed ID: | 31595632 |
| Enhancer experiment: | PCR, Western blot |
| Enhancer experiment description: | The RNA concentration was detected by a microplate reader, and RNA was reverse transcribed to cDNA using the QIAGEN miRNA reverse transcription (miScript II RT Kit) and Thermo reverse transcription kits. Using cDNA as a template, the gene expression of U6, miR-326, C/EBP?, PPAR?, and GAPDH was detected by qPCR. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | The RNA concentration was detected by a microplate reader, and RNA was reverse transcribed to cDNA using the QIAGEN miRNA reverse transcription (miScript II RT Kit) and Thermo reverse transcription kits. Using cDNA as a template, the gene expression of U6, miR-326, C/EBP?, PPAR?, and GAPDH was detected by qPCR. |
About TF
| TF name : | GAPDH |
| TF experiment: | PCR, Western blot |
| TF experiment description: | The RNA concentration was detected by a microplate reader, and RNA was reverse transcribed to cDNA using the QIAGEN miRNA reverse transcription (miScript II RT Kit) and Thermo reverse transcription kits. Using cDNA as a template, the gene expression of U6, miR-326, C/EBP?, PPAR?, and GAPDH was detected by qPCR. |
About Function
| Enhancer function : | The RNA concentration was detected by a microplate reader, and RNA was reverse transcribed to cDNA using the QIAGEN miRNA reverse transcription (miScript II RT Kit) and Thermo reverse transcription kits. Using cDNA as a template, the gene expression of U6, miR-326, C/EBP?, PPAR?, and GAPDH was detected by qPCR. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
The RNA concentration was detected by a microplate reader, and RNA was reverse transcribed to cDNA using the QIAGEN miRNA reverse transcription (miScript II RT Kit) and Thermo reverse transcription kits. Using cDNA as a template, the gene expression of U6, miR-326, C/EBP?, PPAR?, and GAPDH was detected by qPCR. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene