About Enhancer

Enhancer ID: E_01_0223
Species: human
Position : chr10:67881914-67883914
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Obesity
Pubmed ID:  31618980
Enhancer experiment: PCR,Western blot
Enhancer experiment description: SIRT1 couples the deacetylation of various transcription factors and co- factors to the cleavage of NAD+, an indicator of cellular metabolic status, playing a vital role in metabolism, including fat storage, gluconeogenesis, fatty acid oxidation, lipogenesis, insulin secretion, food intake, circadian rhythm, and inflammation [40].

About Target gene

Target gene : --
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: SIRT1 couples the deacetylation of various transcription factors and co- factors to the cleavage of NAD+, an indicator of cellular metabolic status, playing a vital role in metabolism, including fat storage, gluconeogenesis, fatty acid oxidation, lipogenesis, insulin secretion, food intake, circadian rhythm, and inflammation [40].

About TF

TF name : SIRT1
TF experiment: PCR,Western blot
TF experiment description: SIRT1 couples the deacetylation of various transcription factors and co- factors to the cleavage of NAD+, an indicator of cellular metabolic status, playing a vital role in metabolism, including fat storage, gluconeogenesis, fatty acid oxidation, lipogenesis, insulin secretion, food intake, circadian rhythm, and inflammation [40].

About Function

Enhancer function : SIRT1 couples the deacetylation of various transcription factors and co- factors to the cleavage of NAD+, an indicator of cellular metabolic status, playing a vital role in metabolism, including fat storage, gluconeogenesis, fatty acid oxidation, lipogenesis, insulin secretion, food intake, circadian rhythm, and inflammation [40].
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 SIRT1 couples the deacetylation of various transcription factors and co- factors to the cleavage of NAD+, an indicator of cellular metabolic status, playing a vital role in metabolism, including fat storage, gluconeogenesis, fatty acid oxidation, lipogenesis, insulin secretion, food intake, circadian rhythm, and inflammation [40].

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
SIRT1 AndrogenReceptor netpath 167
SIRT1 Circadian Clock reactome 49
SIRT1 E2F transcription factor network pid 77
SIRT1 FoxO family signaling pid 50
SIRT1 HIF-2-alpha transcription factor network pid 34
SIRT1 p53 pathway panther 39
SIRT1 p73 transcription factor network pid 80
SIRT1 Regulation of Androgen receptor activity pid 54
SIRT1 Regulation of HSF1-mediated heat shock response reactome 82
SIRT1 Regulation of retinoblastoma protein pid 67
SIRT1 RORA activates gene expression reactome 27
SIRT1 Signaling events mediated by HDAC Class I pid 89
SIRT1 Signaling events mediated by HDAC Class III pid 41
SIRT1 SIRT1 negatively regulates rRNA Expression reactome 66
SIRT1 Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
SIRT1 Hs_Simplified_Interaction_Map_Between_LOXL4_and_Oxidative_Stress_Pathway_WP3670_90107 wikipathways 14
SIRT1 Hs_Energy_Metabolism_WP1541_91584 wikipathways 13
SIRT1 Hs_Sterol_Regulatory_Element-Binding_Proteins_(SREBP)_signalling_WP1982_90425 wikipathways 15
SIRT1 Hs_NAD_metabolism,_sirtuins_and_aging_WP3630_88178 wikipathways 8
SIRT1 Hs_SREBF_and_miR33_in_cholesterol_and_lipid_homeostasis_WP2011_89947 wikipathways 6

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs