About Enhancer
| Enhancer ID: | E_01_0213 |
| Species: | human |
| Position : | chr2:209421458-209423458   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 31631012 |
| Enhancer experiment: | Flow cytometry,PCR |
| Enhancer experiment description: | Specif_x0002_ically, examination of potential enhancers within these clusters that are located near neural marker genes, MAP2 (Herzog and Weber, 1978) and ROR2 (Endo et al., 2012), found them to be en_x0002_riched for ATAC-seq signal at 1224 h, H3K27ac signal at 4872h, and their expression to peak at 72 h (Figures S2A and S2B). |
About Target gene
| Target gene : | MAP2,NANOG(NANOG),ATF3 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Specif_x0002_ically, examination of potential enhancers within these clusters that are located near neural marker genes, MAP2 (Herzog and Weber, 1978) and ROR2 (Endo et al., 2012), found them to be en_x0002_riched for ATAC-seq signal at 1224 h, H3K27ac signal at 4872h, and their expression to peak at 72 h (Figures S2A and S2B).;Specif_x0002_ically, examination of potential enhancers within these clusters that are located near neural marker genes, MAP2 (Herzog and Weber, 1978) and ROR2 (Endo et al., 2012), found them to be en_x0002_riched for ATAC-seq signal at 1224 h, H3K27ac signal at 4872h, and their expression to peak at 72 h (Figures S2A and S2B).;Pluripotent markers (NANOG and POU5F1) and direct targets of transforming growth factor b (TGF-b) and bone morphogenetic protein (BMP) signaling(SMAD7, ID1, and LEFTY2) were downregulated, and immediate early genes (ATF3, FOS, FOSB, and EGR1/2/3) were transiently upregulated at 3 h, corresponding to the cells stress response against differentiation stimuli.;Pluripotent markers (NANOG and POU5F1) and direct targets of transforming growth factor b (TGF-b) and bone morphogenetic protein (BMP) signaling(SMAD7, ID1, and LEFTY2) were downregulated, and immediate early genes (ATF3, FOS, FOSB, and EGR1/2/3) were transiently upregulated at 3 h, corresponding to the cells stress response against differentiation stimuli.;Pluripotent markers (NANOG and POU5F1) and direct targets of transforming growth factor b (TGF-b) and bone morphogenetic protein (BMP) signaling(SMAD7, ID1, and LEFTY2) were downregulated, and immediate early genes (ATF3, FOS, FOSB, and EGR1/2/3) were transiently upregulated at 3 h, corresponding to the cells stress response against differentiation stimuli. |
About TF
| TF name : | ROR2POU5F1(OCT3,OCT4,OTF-3,OTF3,OTF4,Oct-3,Oct-4) |
| TF experiment: | Flow cytometry,PCR |
| TF experiment description: | Specif_x0002_ically, examination of potential enhancers within these clusters that are located near neural marker genes, MAP2 (Herzog and Weber, 1978) and ROR2 (Endo et al., 2012), found them to be en_x0002_riched for ATAC-seq signal at 1224 h, H3K27ac signal at 4872h, and their expression to peak at 72 h (Figures S2A and S2B).;Specif_x0002_ically, examination of potential enhancers within these clusters that are located near neural marker genes, MAP2 (Herzog and Weber, 1978) and ROR2 (Endo et al., 2012), found them to be en_x0002_riched for ATAC-seq signal at 1224 h, H3K27ac signal at 4872h, and their expression to peak at 72 h (Figures S2A and S2B).;Pluripotent markers (NANOG and POU5F1) and direct targets of transforming growth factor b (TGF-b) and bone morphogenetic protein (BMP) signaling(SMAD7, ID1, and LEFTY2) were downregulated, and immediate early genes (ATF3, FOS, FOSB, and EGR1/2/3) were transiently upregulated at 3 h, corresponding to the cells stress response against differentiation stimuli.;Pluripotent markers (NANOG and POU5F1) and direct targets of transforming growth factor b (TGF-b) and bone morphogenetic protein (BMP) signaling(SMAD7, ID1, and LEFTY2) were downregulated, and immediate early genes (ATF3, FOS, FOSB, and EGR1/2/3) were transiently upregulated at 3 h, corresponding to the cells stress response against differentiation stimuli.;Pluripotent markers (NANOG and POU5F1) and direct targets of transforming growth factor b (TGF-b) and bone morphogenetic protein (BMP) signaling(SMAD7, ID1, and LEFTY2) were downregulated, and immediate early genes (ATF3, FOS, FOSB, and EGR1/2/3) were transiently upregulated at 3 h, corresponding to the cells stress response against differentiation stimuli. |
About Function
| Enhancer function : | Specif_x0002_ically, examination of potential enhancers within these clusters that are located near neural marker genes, MAP2 (Herzog and Weber, 1978) and ROR2 (Endo et al., 2012), found them to be en_x0002_riched for ATAC-seq signal at 1224 h, H3K27ac signal at 4872h, and their expression to peak at 72 h (Figures S2A and S2B). |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Specif_x0002_ically, examination of potential enhancers within these clusters that are located near neural marker genes, MAP2 (Herzog and Weber, 1978) and ROR2 (Endo et al., 2012), found them to be en_x0002_riched for ATAC-seq signal at 1224 h, H3K27ac signal at 4872h, and their expression to peak at 72 h (Figures S2A and S2B). |
About SNP
| SNP ID: | rs2176546 |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| ROR2 | Noncanonical Wnt signaling pathway | pid | 33 |
| ROR2 | PCP/CE pathway | reactome | 21 |
| ROR2 | Wnt | netpath | 118 |
| ROR2 | Wnt signaling network | pid | 29 |
| ROR2 | WNT5A-dependent internalization of FZD2, FZD5 and ROR2 | reactome | 13 |
| ROR2 | Hs_Wnt_Signaling_Pathway_Netpath_WP363_78571 | wikipathways | 28 |
| POU5F1 | HIF-2-alpha transcription factor network | pid | 34 |
| POU5F1 | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| POU5F1 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| POU5F1 | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
| POU5F1 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
| POU5F1 | Hs_Wnt_Signaling_Pathway_and_Pluripotency_WP399_90291 | wikipathways | 12 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene