About Enhancer
| Enhancer ID: | E_01_0212 |
| Species: | human |
| Position : | chr1:23017098-23019098   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Desmosomas |
| Pubmed ID: | 31631026 |
| Enhancer experiment: | Western blot,PCR,Flow cytometry |
| Enhancer experiment description: | We conduct a CRISPR screen revealing that knockout of KDM1A encoding lysine-specific demethylase 1 (LSD1) sensitizes DIPG cells to histone deacetylase (HDAC) inhibitors. Consistently, Corin, a bifunctional inhibitor of HDACs and LSD1, potently inhibits DIPG growth in vitro and in xenografts. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | We conduct a CRISPR screen revealing that knockout of KDM1A encoding lysine-specific demethylase 1 (LSD1) sensitizes DIPG cells to histone deacetylase (HDAC) inhibitors. Consistently, Corin, a bifunctional inhibitor of HDACs and LSD1, potently inhibits DIPG growth in vitro and in xenografts. |
About TF
| TF name : | KDM1A(AOF2,BHC110,CPRF,KDM1,LSD1) |
| TF experiment: | Western blot,PCR,Flow cytometry |
| TF experiment description: | We conduct a CRISPR screen revealing that knockout of KDM1A encoding lysine-specific demethylase 1 (LSD1) sensitizes DIPG cells to histone deacetylase (HDAC) inhibitors. Consistently, Corin, a bifunctional inhibitor of HDACs and LSD1, potently inhibits DIPG growth in vitro and in xenografts. |
About Function
| Enhancer function : | We conduct a CRISPR screen revealing that knockout of KDM1A encoding lysine-specific demethylase 1 (LSD1) sensitizes DIPG cells to histone deacetylase (HDAC) inhibitors. Consistently, Corin, a bifunctional inhibitor of HDACs and LSD1, potently inhibits DIPG growth in vitro and in xenografts. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
We conduct a CRISPR screen revealing that knockout of KDM1A encoding lysine-specific demethylase 1 (LSD1) sensitizes DIPG cells to histone deacetylase (HDAC) inhibitors. Consistently, Corin, a bifunctional inhibitor of HDACs and LSD1, potently inhibits DIPG growth in vitro and in xenografts. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| KDM1A | Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 | reactome | 65 |
| KDM1A | AndrogenReceptor | netpath | 167 |
| KDM1A | Coregulation of Androgen receptor activity | pid | 61 |
| KDM1A | Factors involved in megakaryocyte development and platelet production | reactome | 111 |
| KDM1A | HDACs deacetylate histones | reactome | 94 |
| KDM1A | HDMs demethylate histones | reactome | 49 |
| KDM1A | Notch-mediated HES/HEY network | pid | 48 |
| KDM1A | Notch signaling pathway | pid | 58 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene