About Enhancer
| Enhancer ID: | E_01732 |
| Species: | Homo sapiens |
| Position : | chr12:47483742-47503742   |
| Year: | 2017 |
| Title: | Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | metastatic melanoma |
| PMID: | 29149598 |
| MONDO: | MONDO:0005191 |
| Tissue: | skin |
| Tissue Ontology ID: | UBERON:0002097 |
| Cell Source: | SK-MEL-147 |
| Cell Type: | melanoma cell line; primary/short-term melanoma culture |
| Experiment Type: | super-enhancer constituent validation and endogenous constituent deletion |
| High Throughput Method: | RNA-seq; BRD2 ChIP-seq; BRD4 ChIP-seq; H3K27ac ChIP-seq; H3K4me1 ChIP-seq; ATAC-seq; FOSL2/TEAD4 ChIP-seq; ROSE super-enhancer calling |
| Low Throughput Method: | E1-E5 and E3A-C luciferase assays; CRISPR-Cas9 deletion of E2-E3 and E4-E5; E3A FOSL2/TEAD motif deletion; FOSL2 or TEAD4/TEAD1 perturbation; qRT-PCR; immunoblot; proliferation/apoptosis assays |
About Target Gene
| Target gene : | AMIGO2 |
About TF
| TF name : | FOSL2 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| FOSL2 | AP-1 transcription factor network | pid | 71 |
| FOSL2 | Gene expression of IL2 by AP-1 ( CD4 T cell receptor signaling (JNK cascade) ) | inoh | 5 |
| FOSL2 | Gene expression of IL2 by AP-1 ( CD4 T cell receptor signaling ) | inoh | 5 |
| FOSL2 | JNK cascade ( CD4 T cell receptor signaling (JNK cascade) ) | inoh | 12 |
| FOSL2 | JNK cascade ( CD4 T cell receptor signaling ) | inoh | 10 |
| FOSL2 | Validated transcriptional targets of AP1 family members Fra1 and Fra2 | pid | 37 |
| FOSL2 | Validated transcriptional targets of deltaNp63 isoforms | pid | 47 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
9 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01742 | chr12:47471429-47487762 | skin | malignant cell | melanoma |
| E_01743 | chr12:47471429-47487762 | skin | malignant cell | melanoma |
| E_01733 | chr12:47483742-47503742 | skin | melanoma cell line; primary/short-term melanoma culture | metastatic melanoma |
| E_01734 | chr12:47483742-47503742 | skin | melanoma cell line; primary/short-term melanoma culture | metastatic melanoma |
| E_01735 | chr12:47483742-47503742 | skin | melanoma cell line; primary/short-term melanoma culture | metastatic melanoma |
| E_01736 | chr12:47483742-47503742 | skin | melanoma cell line; primary/short-term melanoma culture | metastatic melanoma |
| E_01737 | chr12:47483742-47503742 | skin | melanoma cell line; primary/short-term melanoma culture | metastatic melanoma |
| E_01738 | chr12:47483742-47503742 | skin | melanoma cell line; primary/short-term melanoma culture | metastatic melanoma |
| E_01739 | chr12:47483742-47503742 | skin | melanoma cell line; primary/short-term melanoma culture | metastatic melanoma |
Expression of Target Genes for the Enhancer