About Enhancer
| Enhancer ID: | E_01613 |
| Species: | Homo sapiens |
| Position : | chr15:99948331-99948831   |
| Year: | 2022 |
| Title: | Lineage-selective super enhancers mediate core regulatory circuitry during adipogenic and osteogenic differentiation of human mesenchymal stem cells. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | obesity |
| PMID: | 36224171 |
| MONDO: | MONDO:0011122 |
| Tissue: | mesenchyme of umbilical cord |
| Tissue Ontology ID: | UBERON:0003422 |
| Cell Source: | Primary human umbilical cord-derived hMSCs after osteogenic differentiation |
| Cell Type: | osteoblast |
| Cell Ontology ID: | CL:0000062 |
| Experiment Type: | Super-enhancer constituent activity assay; TF binding assay; Functional enhancer perturbation assay; Lineage-specific differentiation assay; Disease-variant functional analysis |
| High Throughput Method: | H3K27ac ChIP-seq; DNase-seq; RNA-seq; GWAS |
| Low Throughput Method: | CRISPR-Cas9 deletion of MEF2A-SE functional region FR2; MEF2A ChIP-qPCR; RT-qPCR; ALP staining; dCas9-KRAB CRISPRi |
About Target Gene
| Target gene : | MEF2A |
About TF
| TF name : | MEF2A |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| MEF2A | CDO in myogenesis | reactome | 29 |
| MEF2A | ERK/MAPK targets | reactome | 17 |
| MEF2A | Signaling mediated by p38-alpha and p38-beta | pid | 35 |
| MEF2A | TGF_beta_Receptor | netpath | 220 |
| MEF2A | Hs_p38_MAPK_Signaling_Pathway_WP400_72084 | wikipathways | 28 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
1 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01612 | chr15:99948331-99948831 | mesenchyme of umbilical cord | osteoblast | obesity |
Expression of Target Genes for the Enhancer