About Enhancer
| Enhancer ID: | E_01477 |
| Species: | Homo sapiens |
| Position : | chr21:38576000-38577000   |
| Year: | 2023 |
| Title: | EVI1 drives leukemogenesis through aberrant ERG activation. |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | acute myeloid leukemia |
| PMID: | 36095844 |
| MONDO: | MONDO:0018874 |
| Tissue: | bone marrow |
| Tissue Ontology ID: | UBERON:0002371 |
| Cell Source: | EVI1-high human AML cell models |
| Cell Type: | myeloblast |
| Cell Ontology ID: | CL:0000835 |
| Experiment Type: | TF binding assay; Enhancer reporter assay; Functional perturbation assay; In vivo functional validation |
| High Throughput Method: | MECOM/EVI1 ChIP-seq; H3K27ac profiling; ATAC-seq; RNA-seq; leukemia dependency screening |
| Low Throughput Method: | MECOM/EVI1 ChIP-qPCR; ERG+85 luciferase reporter assay; EVI1 knockdown/withdrawal; ERG knockdown and rescue; RT-qPCR; Western blot; differentiation/colony assays; In vivo phenotyping |
About Target Gene
| Target gene : | ERG |
About TF
| TF name : | MECOM |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| MECOM | PKMTs methylate histone lysines | reactome | 64 |
| MECOM | MAPK signaling pathway | kegg | 264 |
| MECOM | Pathways in cancer | kegg | 321 |
| MECOM | Chronic myeloid leukemia | kegg | 69 |
| MECOM | Hs_White_fat_cell_differentiation_WP3946_90940 | wikipathways | 30 |
Enhancer Associated Network
Overlapping Enhancers (hg38)
1 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01449 | chr21:38576000-38577000 | bone marrow | myeloblast | acute myeloid leukemia |
Expression of Target Genes for the Enhancer