About Enhancer
| Enhancer ID: | E_01475 |
| Species: | Homo sapiens |
| Position : | chr17:39725049-39725326   |
| Year: | 2018 |
| Title: | A novel HER2 gene body enhancer contributes to HER2 expression. |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | breast carcinoma |
| PMID: | 29035388 |
| MONDO: | MONDO:0004989 |
| Tissue: | breast |
| Tissue Ontology ID: | UBERON:0000310 |
| Cell Source: | HCC1954 |
| CVCL ID: | CVCL_1259 |
| Cell Type: | malignant cell |
| Cell Ontology ID: | CL:0001064 |
| Experiment Type: | functional gene-body enhancer-core mapping plus TF and epigenetic validation |
| High Throughput Method: | ENCODE DNase/H3K4me1/H3K27ac integration; TFAP2C ChIP-seq; primary-tumor methylation-expression analysis |
| Low Throughput Method: | serial reporter deletion mapping; luciferase; EMSA/supershift; TFAP2C ChIP-qPCR; TFAP2C siRNA; CRISPR-Cas9 intron-21 editing; dCas9-DNMT3A targeted methylation; bisulfite sequencing; RT-PCR; western blot |
About Target Gene
| Target gene : | ERBB2 |
About TF
| TF name : | TFAP2C |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| TFAP2C | Validated transcriptional targets of TAp63 isoforms | pid | 54 |
Enhancer Associated Network
Overlapping Enhancers (hg38)
4 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01458 | chr17:39725049-39725326 | breast | breast carcinoma cell lines; HEK293T reporter cell | breast carcinoma |
| E_01473 | chr17:39725049-39725326 | breast | malignant cell | breast carcinoma |
| E_01474 | chr17:39725049-39725326 | breast | malignant cell | breast carcinoma |
| E_01479 | chr17:39725049-39725326 | breast | breast carcinoma cell lines; HEK293T reporter cell | breast carcinoma |
Expression of Target Genes for the Enhancer