About Enhancer
| Enhancer ID: | E_01331 |
| Species: | Homo sapiens |
| Position : | chr13:74003796-74004073   |
| Year: | 2022 |
| Title: | A stem cell marker KLF5 regulates CCAT1 via three-dimensional genome structure in colorectal cancer cells. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | colorectal cancer |
| PMID: | 34707247 |
| MONDO: | MONDO:0005575 |
| Tissue: | colon |
| Tissue Ontology ID: | UBERON:0001155 |
| Cell Source: | HT-29 |
| CVCL ID: | CVCL_0320 |
| Cell Type: | malignant cell |
| Cell Ontology ID: | CL:0001064 |
| Experiment Type: | Enhancer activity assay; TF binding assay; Chromatin interaction assay; Functional perturbation assay |
| High Throughput Method: | in vitro enChIP-seq; public H3K27ac ChIP-seq; public BRD4 ChIP-seq; public DNase-seq |
| Low Throughput Method: | CRISPR/Cas9 heterozygous enhancer deletion; PCR and sequencing validation; KLF5 ChIP-qPCR; qRT-PCR; sphere-formation assay; 5-FU and oxaliplatin cell viability assay; flow cytometry; ChIP |
About Target Gene
| Target gene : | KLF5 |
About TF
| TF name : | KLF5 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| KLF5 | Transcriptional regulation of white adipocyte differentiation | reactome | 79 |
| KLF5 | Hs_TGF-beta_Receptor_Signaling_WP560_90187 | wikipathways | 28 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
7 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_00944 | chr13:73880413-74042621 | lung | malignant cell | lung adenocarcinoma |
| E_00945 | chr13:73880413-74042621 | lung | malignant cell | lung adenocarcinoma |
| E_01079 | chr13:73941969-74004429 | head and neck epithelium | malignant cell | head and neck squamous cell carcinoma |
| E_01078 | chr13:74002153-74004429 | head and neck epithelium | malignant cell | head and neck squamous cell carcinoma |
| E_01332 | chr13:74003796-74004073 | colon | malignant cell | colorectal cancer |
| E_01333 | chr13:74003796-74004073 | colon | malignant cell | colorectal cancer |
| E_01408 | chr13:74003796-74004073 | colon | malignant cell | colorectal cancer |
Expression of Target Genes for the Enhancer