About Enhancer
| Enhancer ID: | E_01269 |
| Species: | Homo sapiens |
| Position : | chr3:27713393-27715978   |
| Year: | 2023 |
| Title: | Dynamic network-guided CRISPRi screen identifies CTCF-loop-constrained nonlinear enhancer gene regulatory activity during cell state transitions. |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 37488417 |
| Tissue: | definitive endoderm |
| Tissue Ontology ID: | UBERON:0005439 |
| Cell Source: | HUES 8 |
| CVCL ID: | CVCL_B207 |
| Cell Type: | human embryonic stem cell |
| Experiment Type: | dCas9-KRAB CRISPRi loss-of-function, Hi-C chromatin contact validation |
| High Throughput Method: | ATAC-seq; H3K27ac ChIP-seq; RNA-seq, scRNA-seq, pooled CRISPRi screening; Hi-C |
| Low Throughput Method: | RT-qPCR; FACS flow cytometry, lentiviral transduction, single enhancer clonal CRISPR-Cas9 deletion; CRISPRi |
About Target Gene
| Target gene : | EOMES |
About TF
| TF name : | EOMES |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| EOMES | Downstream signaling in naïve CD8+ T cells | pid | 69 |
| EOMES | IL12-mediated signaling events | pid | 62 |
| EOMES | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
Enhancer Associated Network
Overlapping Enhancers (hg38)
No overlapping enhancers found in hg38 at this locus.
Expression of Target Genes for the Enhancer