About Enhancer
| Enhancer ID: | E_01268 |
| Species: | Homo sapiens |
| Position : | chr18:22061243-22062444   |
| Year: | 2023 |
| Title: | Dynamic network-guided CRISPRi screen identifies CTCF-loop-constrained nonlinear enhancer gene regulatory activity during cell state transitions. |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 37488417 |
| Tissue: | definitive endoderm |
| Tissue Ontology ID: | UBERON:0005439 |
| Cell Source: | HUES 8 |
| CVCL ID: | CVCL_B207 |
| Cell Type: | human embryonic stem cell |
| Experiment Type: | dCas9-KRAB CRISPRi loss-of-function, CRISPR-Cas9 clonal deletion, Hi-C chromatin contact validation |
| High Throughput Method: | ATAC-seq; H3K27ac ChIP-seq; RNA-seq, scRNA-seq, pooled CRISPRi screening; Hi-C |
| Low Throughput Method: | RT-qPCR; FACS flow cytometry, lentiviral transduction, dual clonal enhancer knockout generation; CRISPRi |
About Target Gene
| Target gene : | GATA6 |
About TF
| TF name : | GATA6 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| GATA6 | Factors involved in megakaryocyte development and platelet production | reactome | 111 |
| GATA6 | Notch-mediated HES/HEY network | pid | 48 |
| GATA6 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| GATA6 | Surfactant metabolism | reactome | 30 |
| GATA6 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
Enhancer Associated Network
Overlapping Enhancers (hg38)
No overlapping enhancers found in hg38 at this locus.
Expression of Target Genes for the Enhancer