About Enhancer
| Enhancer ID: | E_01222 |
| Species: | Homo sapiens |
| Position : | chr8:54300591-54300987   |
| Year: | 2023 |
| Title: | Dynamic network-guided CRISPRi screen identifies CTCF-loop-constrained nonlinear enhancer gene regulatory activity during cell state transitions. |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 37488417 |
| Tissue: | inner cell mass |
| Tissue Ontology ID: | UBERON:0000087 |
| Cell Source: | HCC827 |
| CVCL ID: | CVCL_2063 |
| Cell Type: | embryonic stem cell |
| Cell Ontology ID: | CL:0002322 |
| Experiment Type: | CRISPR interference-based functional screen; Endogenous enhancer perturbation; Chromatin interaction assay |
| High Throughput Method: | CRISPRi screen, RNA-seq, ATAC-seq, ChIP-seq, Hi-C; Hi-C; CRISPR genetic screen |
| Low Throughput Method: | RT-qPCR, flow cytometry, FACS; CRISPRi |
About Target Gene
| Target gene : | SOX17 |
About TF
| TF name : | EOMES |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| EOMES | Downstream signaling in naïve CD8+ T cells | pid | 69 |
| EOMES | IL12-mediated signaling events | pid | 62 |
| EOMES | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
Enhancer Associated Network
Overlapping Enhancers (hg38)
4 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01223 | chr8:54300591-54300987 | inner cell mass | embryonic stem cell | NA |
| E_01224 | chr8:54300591-54300987 | inner cell mass | embryonic stem cell | NA |
| E_01225 | chr8:54300591-54300987 | inner cell mass | embryonic stem cell | NA |
| E_01226 | chr8:54300591-54300987 | definitive endoderm | endodermal cell | NA |
Expression of Target Genes for the Enhancer