About Enhancer
| Enhancer ID: | E_01189 |
| Species: | Homo sapiens |
| Position : | chr18:21933810-21935298   |
| Year: | 2023 |
| Title: | Dynamic network-guided CRISPRi screen identifies CTCF-loop-constrained nonlinear enhancer gene regulatory activity during cell state transitions. |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 37488417 |
| Tissue: | definitive endoderm |
| Tissue Ontology ID: | UBERON:0005439 |
| Cell Source: | HUES 8 |
| CVCL ID: | CVCL_B207 |
| Cell Type: | endodermal cell |
| Cell Ontology ID: | CL:0000223 |
| Experiment Type: | Pooled CRISPRi enhancer screening; individual enhancer validation; TF binding; chromatin-state/3D integration; Endogenous enhancer perturbation; Chromatin interaction assay |
| High Throughput Method: | Pooled CRISPRi screen; RNA-seq; scRNA-seq; ATAC-seq; H3K27ac/EOMES/GATA6/SOX17 ChIP-seq; Hi-C; CTCF ChIP-seq; CRISPR genetic screen |
| Low Throughput Method: | Individual CRISPRi; RT-qPCR; flow cytometry/FACS; CRISPRi |
About Target Gene
| Target gene : | GATA6 |
About TF
| TF name : | GATA6 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| GATA6 | Factors involved in megakaryocyte development and platelet production | reactome | 111 |
| GATA6 | Notch-mediated HES/HEY network | pid | 48 |
| GATA6 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| GATA6 | Surfactant metabolism | reactome | 30 |
| GATA6 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
Enhancer Associated Network
Overlapping Enhancers (hg38)
4 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01187 | chr18:21933810-21935298 | inner cell mass | embryonic stem cell | NA |
| E_01188 | chr18:21933810-21935298 | inner cell mass | embryonic stem cell | NA |
| E_01190 | chr18:21933810-21935298 | inner cell mass | embryonic stem cell | NA |
| E_01191 | chr18:21933810-21935298 | inner cell mass | embryonic stem cell | NA |
Expression of Target Genes for the Enhancer