About Enhancer
| Enhancer ID: | E_01182 |
| Species: | Homo sapiens |
| Position : | chr3:111274001-111275000   |
| Year: | 2025 |
| Title: | Enhancer activation from transposable elements in extrachromosomal DNA. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | colorectal adenocarcinoma |
| PMID: | 41120733 |
| MONDO: | MONDO:0005008 |
| Tissue: | sigmoid colon |
| Tissue Ontology ID: | UBERON:0001159 |
| Cell Source: | COLO320DM |
| CVCL ID: | CVCL_0219 |
| Cell Type: | colon epithelial cell, adenocarcinoma-derived |
| Cell Ontology ID: | CL:0011108 |
| Experiment Type: | Enhancer activity assay; endogenous epigenetic perturbation; ecDNA structural mapping; chromatin-interaction mapping; 3D spatial interaction analysis; chromatin-state profiling; transcriptional imaging; cancer-cell fitness assay; Endogenous enhancer perturbation |
| High Throughput Method: | Hi-C; Oxford Nanopore ultra-long-read whole-genome sequencing with CoRAL ecDNA reconstruction; pooled CRISPRi fitness screen with deep sequencing; re-analysis of ATAC-seq; H3K27ac ChIP-seq; BRD4 ChIP-seq |
| Low Throughput Method: | CRISPR-CATCH with PFGE and targeted sequencing; ORCA 3D DNA imaging; DNA-FISH; RNA-FISH; dual-luciferase enhancer assay using MYC and TK promoters; L1PA2/L1M4a1 fragment reporter assays; metaphase-spread ecDNA validation; CRISPRi; CRISPR-CATCH; Reporter assay |
About Target Gene
| Target gene : | MYC |
About TF
| TF name : | NA |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer Associated Network
Overlapping Enhancers (hg19)
1 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01173 | chr3:111274001-111275000 | sigmoid colon | colon epithelial cell, adenocarcinoma-derived | colorectal adenocarcinoma |
Expression of Target Genes for the Enhancer