About Enhancer
| Enhancer ID: | E_01092 |
| Species: | Homo sapiens |
| Position : | chr4:122542425-122542887   |
| Year: | 2025 |
| Title: | Bidirectional epigenetic editing reveals hierarchies in gene regulation. |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 38760566 |
| Tissue: | blood |
| Tissue Ontology ID: | UBERON:0000178 |
| Cell Source: | Activated primary human CD4+ T cells |
| Cell Type: | CD4-positive, alpha-beta T cell |
| Cell Ontology ID: | CL:0000624 |
| Experiment Type: | Enhancer activity assay; TF binding assay; Functional perturbation assay; Enhancer-promoter regulatory interaction analysis; Endogenous enhancer perturbation; Disease-variant functional analysis |
| High Throughput Method: | Pooled CRISPRi/CRISPRai gRNA enrichment sequencing; public JUNB ChIP-seq; public histone ChIP-seq; published ATAC-seq; ABC-model enhancer-promoter contact analysis using public Hi-C; single-cell RNA-seq; RNA-seq |
| Low Throughput Method: | Individual CRISPRi; intracellular IL2 flow cytometry; CRISPRi; CRISPRa |
About Target Gene
| Target gene : | IL2 |
About TF
| TF name : | JUNB |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| JUNB | AP-1 transcription factor network | pid | 71 |
| JUNB | ATF-2 transcription factor network | pid | 59 |
| JUNB | BCR | netpath | 161 |
| JUNB | Calcineurin-regulated NFAT-dependent transcription in lymphocytes | pid | 50 |
| JUNB | Calcium signaling in the CD4+ TCR pathway | pid | 29 |
| JUNB | Downstream signaling in naïve CD8+ T cells | pid | 69 |
| JUNB | IL6-mediated signaling events | pid | 48 |
| JUNB | Inflammation mediated by chemokine and cytokine signaling pathway | panther | 189 |
| JUNB | SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription | reactome | 32 |
| JUNB | TGF-beta signaling pathway | panther | 88 |
| JUNB | TGF_beta_Receptor | netpath | 220 |
| JUNB | TNFalpha | netpath | 274 |
| JUNB | Validated transcriptional targets of AP1 family members Fra1 and Fra2 | pid | 37 |
| JUNB | Hs_Kit_receptor_signaling_pathway_WP304_78799 | wikipathways | 46 |
| JUNB | Hs_TGF-beta_Signaling_Pathway_WP366_90028 | wikipathways | 67 |
Enhancer Associated Network
Overlapping Enhancers (hg38)
1 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01091 | chr4:122542425-122542887 | blood | CD4-positive, alpha-beta T cell | NA |
Expression of Target Genes for the Enhancer