About Enhancer
| Enhancer ID: | E_00042 |
| Species: | Homo sapiens |
| Position : | chr21:36179867-36180107   |
| Year: | 2020 |
| Title: | Disruption of super-enhancer-driven tumor suppressor gene RCAN1.4 expression promotes the malignancy of breast carcinoma. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | breast cancer |
| PMID: | 32771023 |
| MONDO: | MONDO:0007254 |
| Tissue: | breast |
| Tissue Ontology ID: | UBERON:0000310 |
| Cell Source: | MDA-MB-231 |
| CVCL ID: | CVCL_0062 |
| Cell Type: | mammary gland epithelial cell |
| Cell Ontology ID: | CL:0002327 |
| Experiment Type: | Enhancer activity assay; TF binding assay; Functional perturbation assay; Endogenous enhancer perturbation; Chromatin-state profiling; In vivo functional validation |
| High Throughput Method: | H3K27ac ChIP-seq; H3K4me1 ChIP-seq; Hi-C; ChIP-seq |
| Low Throughput Method: | Dual-luciferase reporter assay; RUNX3 ChIP-qPCR; RUNX3 siRNA knockdown; RUNX3 motif mutagenesis; double-CRISPR enhancer deletion; RT-qPCR; Western blot; CRISPR-Cas9 endogenous deletion/editing; Luciferase reporter assay; ChIP-qPCR; ChIP; In vivo phenotyping; Reporter assay |
About Target Gene
| Target gene : | RCAN1 |
About TF
| TF name : | RUNX3 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| RUNX3 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| RUNX3 | TGF_beta_Receptor | netpath | 220 |
| RUNX3 | Hs_TYROBP_Causal_Network_WP3945_90843 | wikipathways | 54 |
| RUNX3 | Hs_Sudden_Infant_Death_Syndrome_(SIDS)_Susceptibility_Pathways_WP706_86078 | wikipathways | 33 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
3 found
Expression of Target Genes for the Enhancer