Sample ID: | Sample_0008 |
Biosample name: | iMeLCs induced from primed UCLA1 hESCs |
Biosample type: | Stem Cell |
Tissue type: | Embryo |
Cancer type: | Normal |
Region number: | 65001 |
Length: | 34707.324 kb |
GEO/SRA ID: | SRR7942729 |
Mean insert size: | 156.952 |
Standard deviation: | 108.292 |
TSS enrichment score: | 27.89620 |
FRiP: | 0.17247 |
Diasgnostic plot | |
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Plot A: a pie chart of annotated genomic features. |
Note A: the proportion of annotating genomic region (Promoter, 5'UTR, 3'UTR, Exon, Intron, Downstream and Intergenic). |
Plot B: a heatmap of peaks binding to TSS region. |
Note B: the profile of ATAC peaks binding to TSS region (+/- 1kb). |
Read mapping: | bowtie2(v2.2.5) -p 10 -X 2000 -x -S |
Duplicates Removing: | picard MarkDuplicates REMOVE_DUPLICATES=true ASSUME_SORTED=true |
Peak calling: | macs2(2.1.2) --broad--SPMR --nomodel --extsize 200 -q 0.01 |
Trimming the adapter: | trim_galore --phred33 --stringency 3 --length 20 |