About Enhancer
| Enhancer ID: | E_02_305 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr2:130280565-130281887   |
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| Biosample name: | C2C12,MEFs | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | <2KB | |
| Pubmed ID: | 26774474 |
| Enhancer experiment: | ChIP-qPCR,ChIP-seq,ChIP,EMSA,Luciferase Reporter Assay,qPCR | |
| Enhancer experiment description: | Interactions of methylated PGC-1a[K779me] with the Spt-Ada_x0002_Gcn5-acetyltransferase (SAGA) complex, the Medi_x0002_ator members MED1 and MED17, and the NOP2/ Sun RNA methytransferase 7 (NSUN7) reinforce tran_x0002_scription, and are concomitant with the m5C mark on enhancer RNAs (eRNAs). |
About Target gene
| Target gene : | Idh3b(C78231) | |
| Strong evidence: | -- | |
| Less strong evidence: | qPCR | |
| Target gene experiment description: | Direct knockdown studies combined with ChIP analysis revealed some bona fide targets as Pfkl,Sirt5,Idh3b,and Hmox2 genes.Although a significant pool of PGC-1a[K779me] resides within the TSS, we were able to localize a significant binding at enhancer regions that allowed the characterization of eRNAs overlapping those PGC-1a[K779me] peaks. Thus, complex ncRNA-protein interactions appear consequential to the fidelity of transcription and to the cellular metabolic fate. |
About TF
| TF name : | TfiidLxr(Lxr)nrf2Ppar | |
| TF experiment: | ChIP-seq | |
| TF experiment description: | urthermore, DNA motifs overrepresented in PGC-1α[K779me] fell into four dominant motif consensus classes, including the TFIID, LXR, NRF2, and the PPAR family of transcription factors (Figure 3F, left and middle), which were localized in four metabolic genes identified as specific targets of Pgc-1α by knockdown experiments (Figure 3F, right; Figure S3A): 6-phosphofructokinase (Pfkl), Sirtuin 5 (Sirt5), isocitrate dehydrogenase 3 (Idh3b), and Heme oxygenase (decycling) 2 (Hmox2).Thus, depletion of Pgc-1α led to a decrease of Pfkl, Sirt5, Idh3b, and Hmox2 expression, which was rescued with wild-type PGC-1α, but not mutated PGC-1α[K779R], overexpression. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
