Enhancer ID: | E_02_302 | |
Enhancer symbol: | MCE | |
Species: | Mouse | |
Position : | chr17:26829232-26829742 |
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Biosample name: | Cardiac Progenitor Cell | |
Experiment class : | Low throughput |
Enhancer type: | enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2kb | |
Pubmed ID: | 26787680 |
Enhancer experiment: | Luciferase Reporter Assay,Transient Transfection Assay | |
Enhancer experiment description: | The influence of desmin on nkx2.5 gene expression was monitored first with the luciferase (LUC) reporter plasmid pNKE24 (Searcy et al., 1998), containing the proximal enhancerand promoter region (PEPR) of the nkx2.5 gene and then with the LUC reporter plasmid pMCE, containing the minimal cardiac specific enhancer region(MCE) (Lien et al., 1999) in addition to the PEPR (Fig. 1A; for precise localization along the nkx2.5 gene see Fig. 4A).Co-transfection of 10T1/2 fibroblasts with pNKE24 and a desmin-expressing plasmid (desmin-ect.) resulted in a significant decrease of the LUC activity (Fig. 1B). |
Target gene : | Nkx2-5(CHNG5,CSX,CSX1,HLHS2,NKX2.5,NKX2E,NKX4-1,VSD3) | |
Strong evidence: | -- | |
Less strong evidence: | Luciferase Reporter Assay,PCR,ChIP-seq | |
Target gene experiment description: | The influence of desmin on nkx2.5 gene expression was monitored first with the luciferase (LUC) reporter plasmid pNKE24 (Searcy et al., 1998), containing the proximal enhancerand promoter region (PEPR) of the nkx2.5 gene and then with the LUC reporter plasmid pMCE, containing the minimal cardiac specific enhancer region(MCE) (Lien et al., 1999) in addition to the PEPR (Fig. 1A; for precise localization along the nkx2.5 gene see Fig. 4A).Co-transfection of 10T1/2 fibroblasts with pNKE24 and a desmin-expressing plasmid (desmin-ect.) resulted in a significant decrease of the LUC activity (Fig. 1B). |
TF name : | Desmin(Desmin) | |
TF experiment: | ChIP,PCR | |
TF experiment description: | Desmin is a component of transcription factor complexes interacting with regulatory regions of the nkx2.5 gene at the beginning of cardiomyogenesis. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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