About Enhancer

Enhancer ID: E_02_302
Enhancer symbol: MCE
Species: Mouse
Position : chr17:26829232-26829742
Biosample name: Cardiac Progenitor Cell
Experiment class : Low throughput
Enhancer type: enhancer
Disease: --
DO: --
Mesh: --
Distance from TSS: >2kb
Pubmed ID:  26787680
Enhancer experiment: Luciferase Reporter Assay,Transient Transfection Assay
Enhancer experiment description: The influence of desmin on nkx2.5 gene expression was monitored first with the luciferase (LUC) reporter plasmid pNKE24 (Searcy et al., 1998), containing the proximal enhancerand promoter region (PEPR) of the nkx2.5 gene and then with the LUC reporter plasmid pMCE, containing the minimal cardiac specific enhancer region(MCE) (Lien et al., 1999) in addition to the PEPR (Fig. 1A; for precise localization along the nkx2.5 gene see Fig. 4A).Co-transfection of 10T1/2 fibroblasts with pNKE24 and a desmin-expressing plasmid (desmin-ect.) resulted in a significant decrease of the LUC activity (Fig. 1B).

About Target gene

Target gene : Nkx2-5(CHNG5,CSX,CSX1,HLHS2,NKX2.5,NKX2E,NKX4-1,VSD3)
Strong evidence: --
Less strong evidence: Luciferase Reporter Assay,PCR,ChIP-seq
Target gene experiment description: The influence of desmin on nkx2.5 gene expression was monitored first with the luciferase (LUC) reporter plasmid pNKE24 (Searcy et al., 1998), containing the proximal enhancerand promoter region (PEPR) of the nkx2.5 gene and then with the LUC reporter plasmid pMCE, containing the minimal cardiac specific enhancer region(MCE) (Lien et al., 1999) in addition to the PEPR (Fig. 1A; for precise localization along the nkx2.5 gene see Fig. 4A).Co-transfection of 10T1/2 fibroblasts with pNKE24 and a desmin-expressing plasmid (desmin-ect.) resulted in a significant decrease of the LUC activity (Fig. 1B).

About TF

TF name : Desmin(Desmin)
TF experiment: ChIP,PCR
TF experiment description: Desmin is a component of transcription factor complexes interacting with regulatory regions of the nkx2.5 gene at the beginning of cardiomyogenesis.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:
--

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs