About Enhancer

Enhancer ID: E_02_297
Enhancer symbol: --
Species: Mouse
Position : chr8:120876144-120876856
Biosample name: Mouse Embryos
Experiment class : Low+High throughput
Enhancer type: Enhancer
Disease: --
DO: --
Mesh: --
Distance from TSS: >2kb
Pubmed ID:  27166834
Enhancer experiment: 3C,ChIP-seq
Enhancer experiment description: To identify the presence of physical interaction between the 11 enhancer candidates and the Foxf1 promoter, 3C assays were performed. Among 11 selected regions, three enhancer candidates demonstrated interactions with the Foxf1 promoter.

About Target gene

Target gene : Foxf1(ACDMPV,FKHL5,FREAC1)
Strong evidence: 3C
Less strong evidence: RT-qPCR,DNaseI-seq,Transgenic mice
Target gene experiment description: To investigate the in vivo activity of the discovered enhanc_x0002_ers and compare the location of activity with Foxf1-express_x0002_ing regions, we generated enhancer-driven lacZ-reporter mice 5′e207mFoxf1-lacZ and 5′e201mFoxf1-lacZ.Foxf1 mRNA was expressed in the maxilla, mandible, limbs, sclerotome and gut of wild-type mouse embryos at E12 (Fig. 2a), whereas lacZ-positive cells were observed in part of the forebrain and neural tube in 5′e207mFoxf1-lacZ transgenic mice at E11 and E12 as well as 5′e201mFoxf1-lacZ transgenic mouse embryos at E11 after x-gal staining.Foxf1 was also expressed in the mesenchyme surrounding epithelial buds in lungs at E12.

About TF

TF name : Gli1(AV235269,Zfp-5,Zfp5)Gli2(Gli2)Gli3
TF experiment: RT-qPCR,Luciferase Reporter Assay
TF experiment description: Schematic drawing of luciferase reporter constructs containing 112 bp 5′e207mFoxf1 and 173 bp 5′e201mFoxf1 fragments with Gli binding sites is shown. Luciferase reporter constructs were assayed for luciferase activity without or with Gli1 over-expression vector at 50 or 100 ng.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:
--

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs