About Enhancer
| Enhancer ID: | E_02_290 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr12:103852772-103852898   |
|
| Biosample name: | Hepatoma Cells | |
| Experiment class : | low throughput |
| Enhancer type: | enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | -- | |
| Pubmed ID: | 2835657 |
| Enhancer experiment: | Transient Assay,EMSA | |
| Enhancer experiment description: | To test whether the more distant upstream sequences could function as an enhancer, the -523- to -168-bp restriction fragment was attached in either orientation (at -341 bp)to a mouse ,beta-globin promoter.The results of transfection experiments showed a 10- to 15-fold enhancement of beta-globin transcription when this region was present in either orientation . |
About Target gene
| Target gene : | Serpina1a | |
| Strong evidence: | -- | |
| Less strong evidence: | Transient Assay,EMSA | |
| Target gene experiment description: | Proteins present in nuclear extracts bind to the functionally defined elements present within the al-AT enhancer. Nuclear extracts from Hep-G2 cells were incubated with end-labeled DNA probes from the enhancer region to detect nuclear protein-binding sites by the gel retardation or gel shift assay. Each protein-binding site correlated with a functionally importantregion necessary for full enhancer activity. These experiments demonstrated a complex arrangement of regulatory elements comprising the al-antitrypsin enhancer. |
About TF
| TF name : | -- | |
| TF experiment: | -- | |
| TF experiment description: | -- |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
