Enhancer ID: | E_02_277 | |
Enhancer symbol: | miR-1 SE | |
Species: | Mouse | |
Position : | chr2:180362582-180377003 |
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Biosample name: | myotubes | |
Experiment class : | Low+High throughput |
Enhancer type: | Super-Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | -- | |
Pubmed ID: | 26855180 |
Enhancer experiment: | CRISPR/Cas9,RT-qPCR | |
Enhancer experiment description: | We performed a functional dissection ofmiRNA SEs in vivo by generating cell lines depleted of individual miRNA SE constituents (about 400–700 bp) in multiple cell types through a CRISPR/Cas9-based approach (Table S5). We analyzed three miRNA SEs: miR-290-295 SE in mESCs, miR-1 SE in myotubes, and miR-148a SE in Pro-B cells. In the C2C12 cell line, a model for myotube differentiation, SE was only observed for one of the two miR-1 genes (miR-1a-1/133a-2) and myotube differentiation strongly induced primary miRNAs of only this miRNA gene (Fig-ure S5B).Deletion of all four constituents of miR-1 SE dramatically suppressed induction of miR-1 and miR-133 and myogenic differentiationmarkers such asMyogenin andmuscle creatine kinase (MCK), phenocopying miR-1 knockdown phenotype (Chen et al., 2006)(Figures 4C and4D and S5C). |
Target gene : | MiR-1a-1(Mir1-1,Mirn1b,Mirn1c,Mirn1d,Mirn1-1,mir-1a-1,mmu-mir-1-1,mmu-mir-1a-1),MiR-133a-2(Mirn133a-2,mir-133a-2) | |
Strong evidence: | CRISPR/Cas9 | |
Less strong evidence: | RT-qPCR | |
Target gene experiment description: | We performed a functional dissection ofmiRNA SEs in vivo by generating cell lines depleted of individual miRNA SE constituents (about 400–700 bp) in multiple cell types through a CRISPR/Cas9-based approach (Table S5). We analyzed three miRNA SEs: miR-290-295 SE in mESCs, miR-1 SE in myotubes, and miR-148a SE in Pro-B cells. In the C2C12 cell line, a model for myotube differentiation, SE was only observed for one of the two miR-1 genes (miR-1a-1/133a-2) and myotube differentiation strongly induced primary miRNAs of only this miRNA gene (Fig-ure S5B).Deletion of all four constituents of miR-1 SE dramatically suppressed induction of miR-1 and miR-133 and myogenic differentiationmarkers such asMyogenin andmuscle creatine kinase (MCK), phenocopying miR-1 knockdown phenotype (Chen et al., 2006)(Figures 4C and4D and S5C). |
TF name : | -- | |
TF experiment: | -- | |
TF experiment description: | -- |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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