Enhancer ID: | E_02_270 | |
Enhancer symbol: | miR-290-295 SE | |
Species: | Mouse | |
Position : | chr7:3193003-3218182 |
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Biosample name: | Embryonic Stem Cell | |
Experiment class : | Low+High throughput |
Enhancer type: | Super-Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | -- | |
Pubmed ID: | 26855180 |
Enhancer experiment: | CRISPR/Cas9,RT-qPCR | |
Enhancer experiment description: | We performed a functional dissection of miRNA SEs in vivo by generating cell lines depleted of individual miRNA SE constituents (about 400–700 bp) in multiple cell types through a CRISPR/Cas9-based approach (Table S5). We analyzed three miRNA SEs: miR-290-295 SE in mESCs, miR-1 SE in myotubes, and miR-148a SE in Pro-B cells. Among seven constituents of miR-290-295 SE (E1-E7, Figure 4A), deletion of individual elements except for E6 led to substantial decrease (ranging from 50% to 80%) in de novo production of mature miRNAs in TT-FHAgo2 mESC background without concomitant changes in other miRNA production (Figures 4B and S5A). A 5-fold reduction in expression upon deletion of one constituent of a group of six indicates cooperative interactions.As expected from a decrease in these miRNAs, these deletions also suppressed expression of Ago2-inducible gene Lin28a, a regulator of ES cells (Figure 4B). By comparing pri-miRNAs, pre-miRNAs, andmaturemiRNAs levels, we found that deletion of several miR-290-295 SE constituents modestly (2 to 3 fold) suppressed pri-miRNA production and further attenuated the ratio of pre-miRNA and mature miRNA to pri-miRNA (2 to 3 fold) (Figure 4G). This trend was also observed formiR-1 SE inmyotubes (Figure 4H). |
Target gene : | MiR-290(Mirn290,mir-290a,mmu-miR-290,mmu-mir-290a),291a,292,291b,293,294,295-295 | |
Strong evidence: | CRISPR/Cas9 | |
Less strong evidence: | RT-qPCR | |
Target gene experiment description: | We performed a functional dissection of miRNA SEs in vivo by generating cell lines depleted of individual miRNA SE constituents (about 400–700 bp) in multiple cell types through a CRISPR/Cas9-based approach (Table S5). We analyzed three miRNA SEs: miR-290-295 SE in mESCs, miR-1 SE in myotubes, and miR-148a SE in Pro-B cells. Among seven constituents of miR-290-295 SE (E1-E7, Figure 4A), deletion of individual elements except for E6 led to substantial decrease (ranging from 50% to 80%) in de novo production of mature miRNAs in TT-FHAgo2 mESC background without concomitant changes in other miRNA production (Figures 4B and S5A). A 5-fold reduction in expression upon deletion of one constituent of a group of six indicates cooperative interactions.As expected from a decrease in these miRNAs, these deletions also suppressed expression of Ago2-inducible gene Lin28a, a regulator of ES cells (Figure 4B). By comparing pri-miRNAs, pre-miRNAs, andmaturemiRNAs levels, we found that deletion of several miR-290-295 SE constituents modestly (2 to 3 fold) suppressed pri-miRNA production and further attenuated the ratio of pre-miRNA and mature miRNA to pri-miRNA (2 to 3 fold) (Figure 4G). This trend was also observed formiR-1 SE inmyotubes (Figure 4H). |
TF name : | -- | |
TF experiment: | -- | |
TF experiment description: | -- |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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