Enhancer ID: | E_02_265 | |
Enhancer symbol: | Enhancer N2 | |
Species: | Mouse | |
Position : | chr3:34645597-34646134 |
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Biosample name: | Mouse Embryos | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 21185279 |
Enhancer experiment: | ChIP | |
Enhancer experiment description: | ChIP assay demonstrating Pou5f1 binding to the Enhancer N2 region.In transgenic mouse embryos, mouse enhancer N2 showed activity in the entire epiblast at E6.5, and ANP area at E7.75, as indicated by the expression of the N2-hspLacZ transgene, coincident with Sox2 expression in the epiblast and forming ANP. |
Target gene : | Sox2(ANOP3,MCOPS3) | |
Strong evidence: | -- | |
Less strong evidence: | ChIP,RT-PCR | |
Target gene experiment description: | ChIP assay demonstrating Pou5f1 binding to the Enhancer N2 region. Embryos were injected with pou5f1-FLAG mRNA or left non-injected, and ChIP was performed using anti-FLAG antibody. qPCR analysis shows enrichment of the Enhancer N2 region sequence (N2) in injected embryos without enrichment of the control region (Rpl5b). |
TF name : | Pou5f1(OCT3,OCT4,OTF-3,OTF3,OTF4,Oct-3,Oct-4) | |
TF experiment: | EMSA | |
TF experiment description: | These observations indicated that simultaneous binding of POU factors to the core sequence is essential for eliciting enhancer N2 activity in all examined developmental stages of the mouse embryos.these EMSA data in conjunction with the enhancer activity analyses shown in Fig. 3 indicate that the simultaneous binding of POU factors to POU-1 and POU-2/3 sites is required for the activation of the 176-bp enhancer in ES, epiblast/EpiSC and ANP cells. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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