About Enhancer

Enhancer ID: E_02_216
Enhancer symbol: --
Species: Mouse
Position : chr17:45790442-45800636
Biosample name: Primary Mouse Embryonic Fibroblasts
Experiment class : Low+High throughput
Enhancer type: Enhancer
Disease: --
DO: --
Mesh: --
Distance from TSS: >2KB
Pubmed ID:  29272704
Enhancer experiment: ChIP-seq,ATAC-seq
Enhancer experiment description: We mapped cell identity and LRG enhancers from Spret/EiJ MEFs (ATAC-seq, H3K4me1/2 ChIP-seq, ATAC-seq) to identify strain-specific cell identity and LRG enhancers (Figure 2a,b). We focused on cases in which there is no longer an ATAC-seq peak in the strain in which the enhancer sequence is no longer functional (see methods), reasoning that such examples would be most useful for determining which TF binding motifs are required for enhancer selection. In total, we identified 42 LRG enhancers and 363 cell identity enhancers that were selected in a strain-specific manner (Figure 2b).

About Target gene

Target gene : Vegfa(MVCD1,VEGF,VPF)
Strong evidence: --
Less strong evidence: ChIP-seq,PCR
Target gene experiment description: Representative LRG Enhancers at the locus of the LRG Spred2. Scale bars indicate normalized read densities for each ChIP-seq and shaded boxes denote LRG Enhancers.To determine whether BAF is involved in inducible nucleosome remodeling at LRG enhancers, we performed ChIP-seq for Smarca4, a core component of BAF to determine if the complex is recruited to AP-1-bound LRG enhancers upon stimulation. This revealed that BAF levels are low at most LRG enhancers prior to stimulation but increase significantly upon stimulation (Figure 7a,b).

About TF

TF name : Jun(AP-1,AP1,c-Jun,cJUN,p39)
TF experiment: ChIP-seq,ATAC-seq
TF experiment description: To identify fibroblast LDTFs that might be required for targeting AP-1 to fibroblast-specific Enhancers, we first performed ChIP-seq for the AP-1 TFs Fos/Jund in SPRET/EiJ MEFs to identify all strain-specific sites of AP-1 binding (n=1,224; Figure 5f). We next excluded all the strain-specific binding sites at which there was a SNP within an AP-1 motif that could directly explain the observed loss of AP-1 binding in one of the two strains (~47% of strain-specific AP-1 bound sites) (Figure 5g).

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:
--

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs