Enhancer ID: | E_02_206 | |
Enhancer symbol: | -- | |
Species: | Mouse | |
Position : | chr2:103887795-103888195 |
|
Biosample name: | Embryo | |
Experiment class : | Low+High throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 29141987 |
Enhancer experiment: | ChIP-seq | |
Enhancer experiment description: | We overlapped TBS with genome‐wide H3K4me1 peaks from T WT mesodermal cells and found the same trend of decreased H3K27ac within ±1 kb of TBS/H3K4me1 sites (Fig EV4E), suggesting that T functions to coordinate H3K27ac at putative enhancer regions throughout the genome, and may regulate the switch between poised H3K4me1(+) enhancers and active H3K27ac/H3K4me1(+) enhancer regions. |
Target gene : | Lmo2(Rbtn-2,Rbtn2,Rhom-2,Ttg2) | |
Strong evidence: | -- | |
Less strong evidence: | RT-qPCR,ChIP-qPCR,ChIP-seq | |
Target gene experiment description: | Both the −70 enhancer and the TSS of Lmo2 display a decrease in H3K27ac in T Y88A mutant mesodermal cells, in the ChIP‐Seq data and confirmed by ChIP‐qPCR (Figs (Figs5A5A and B, and EV5A). We reasoned that T binding to the −70 enhancer of Lmo2 may recruit permissive chromatin marks to the TSS to regulate expression, and that this regulation is disrupted in T Y88A mutant mesodermal cells. |
TF name : | T(Bra,D17Mit170,Low,Lr1,Tbxt,Tl2,Tl3,cou,me75,T) | |
TF experiment: | ChIP-seq,ChIP-qPCR | |
TF experiment description: | We performed a co-immunoprecipitation analysis from in vitrodifferentiated mesodermal cells and found an interaction between endogenous T and p300.Strikingly, loss of H3K27ac was found at TBS upstream of the T genomic locus itself by ChIP-Seq . We validated this decrease in H3K27ac at the T genomic locus by ChIP-qPCR in TY88A and TWT mesodermal cells differentiated in vitro. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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