About Enhancer

Enhancer ID: E_02_174
Enhancer symbol: --
Species: Mouse
Position : chr7:35156353-35161233
Biosample name: Myeloid Cells
Experiment class : Low+High throughput
Enhancer type: Enhancer
Disease: --
DO: --
Mesh: --
Distance from TSS: >2KB
Pubmed ID:  26937964
Enhancer experiment: ChIP-seq,CRISPR/Cas9,Transgenic mice
Enhancer experiment description: To determine the effect of deleting the enhancer in vivo, we now characterize C57BL/6 mice in which loxP sites flank a 688 bp DNA segment containing the enhancer.

About Target gene

Target gene : Cebpa(C/ebpalpha,CBF-A,Cebp)
Strong evidence: CRISPR/Cas9
Less strong evidence: ChIP-seq
Target gene experiment description: These findings demonstrate a critical role for the +37 kb Cebpa Enhancer for hematopoietic-specific Cebpa expression,with Enhancer deletion leading to impaired myelopoiesis and potentially preleukemic progenitor expansion.

About TF

TF name : Runx1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha)
TF experiment: ChIP-seq
TF experiment description: Runx1,C/EBPα,Spi1,Erg,Fli-1,GATA2,Scl,Meis1,and Gfi-1b bind chromatin in the region of this Enhancer in hematopoietic cells as determined by ChIP-Seq.

About Function

Enhancer function : These findings demonstrate a critical role for the +37 kb Cebpa enhancer for hematopoietic-specific Cebpa expression, with enhancer deletion leading to impaired myelopoiesis and potentially preleukemic progenitor expansion.
Enhancer function experiment: CRISPR/Cas9,PCR,Western blot
Enhancer function
experiment description:
Together, these data indicate that reduced Cebpa expression consequent to +37 kb Enhancer deletion impairs hematopoietic cell autonomous granulopoiesis in vitro, leading to preservation of immature myeloid progenitors capable of long-term, IL-3-dependent proliferation without complete terminal maturation,a preleukemic phenotype.

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs