Enhancer ID: | E_02_174 | |
Enhancer symbol: | -- | |
Species: | Mouse | |
Position : | chr7:35156353-35161233 |
|
Biosample name: | Myeloid Cells | |
Experiment class : | Low+High throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 26937964 |
Enhancer experiment: | ChIP-seq,CRISPR/Cas9,Transgenic mice | |
Enhancer experiment description: | To determine the effect of deleting the enhancer in vivo, we now characterize C57BL/6 mice in which loxP sites flank a 688 bp DNA segment containing the enhancer. |
Target gene : | Cebpa(C/ebpalpha,CBF-A,Cebp) | |
Strong evidence: | CRISPR/Cas9 | |
Less strong evidence: | ChIP-seq | |
Target gene experiment description: | These findings demonstrate a critical role for the +37 kb Cebpa Enhancer for hematopoietic-specific Cebpa expression,with Enhancer deletion leading to impaired myelopoiesis and potentially preleukemic progenitor expansion. |
TF name : | Runx1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha) | |
TF experiment: | ChIP-seq | |
TF experiment description: | Runx1,C/EBPα,Spi1,Erg,Fli-1,GATA2,Scl,Meis1,and Gfi-1b bind chromatin in the region of this Enhancer in hematopoietic cells as determined by ChIP-Seq. |
Enhancer function : | These findings demonstrate a critical role for the +37 kb Cebpa enhancer for hematopoietic-specific Cebpa expression, with enhancer deletion leading to impaired myelopoiesis and potentially preleukemic progenitor expansion. |
Enhancer function experiment: | CRISPR/Cas9,PCR,Western blot |
Enhancer function experiment description: |
Together, these data indicate that reduced Cebpa expression consequent to +37 kb Enhancer deletion impairs hematopoietic cell autonomous granulopoiesis in vitro, leading to preservation of immature myeloid progenitors capable of long-term, IL-3-dependent proliferation without complete terminal maturation,a preleukemic phenotype. |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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