Enhancer ID: | E_02_172 | |
Enhancer symbol: | -- | |
Species: | Mouse | |
Position : | chr14:69221635-69222851 |
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Biosample name: | G1ER Erythroid Cell | |
Experiment class : | Low+High throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 26766440 |
Enhancer experiment: | CRISPR/Cas9,ChIP-seq | |
Enhancer experiment description: | Specifically, we analyzed active enhancer-associated histone modifications H3K4me1 and H3K27ac by chromatin immunoprecipitation sequencing (ChIP-seq) and transcriptomic profiles in four distinct populations of human HSPCs or ProEs at fetal or adult stage (Figure 1B-E; Figure S1A).Identification of lineage or developmental stage-specific enhancers. Venn diagram shows the overlap between HSPC and ProE, or fetal and adult enhancers. The numbers of lost, shared, or gained enhancers in each comparison are shown. |
Target gene : | Slc25a37(HT015,MFRN,MFRN1,MSC,MSCP,PRO1278,PRO1584,PRO2217) | |
Strong evidence: | -- | |
Less strong evidence: | ChIP-seq,ChIP-qPCR | |
Target gene experiment description: | Loss of E3 leads to near absence of H3K27ac, GATA1 and TAL1 occupancy at the neighboring E1 and E2 Enhancers, whereas loss of E1 or E2 has minimal impact on H3K27ac or GATA1/TAL1 binding at neighboring Enhancers (Figure 3D-G). These results strongly suggest that, despite the indistinguishable chromatin features and TF occupancy at the Slc25a37 constituent Enhancers, the E3 Enhancer is functionally more potent than its neighboring Enhancers in directing transcriptional activation. |
TF name : | Gata1(ERYF1,GATA-1,GF-1,GF1,NF-E1,NFE1,XLANP,XLTDA,XLTT)Gata2(DCML,IMD21,MONOMAC,NFE1B) | |
TF experiment: | ChIP-seq,CRISPR/Cas9 | |
TF experiment description: | To further dissect the role of GATA switch in enhancer turnover, we compared GATA2 and GATA1 occupancy within A0 and A5 enhancers by ChIP-seq. Specifically, we enumerated the distribution of enhancers occupied by GATA2-Only, GATA1-Only, or GATA2-to-GATA1 switch.We then employed genomic editing to dissect the requirement of GATA switch enhancers within the paradigmatic Gata2 locus. |
Enhancer function : | GATA2-to-GATA1 switch is prevalent at dynamic enhancers and drives erythroid enhancer commissioning. |
Enhancer function experiment: | CRISPR/Cas9 |
Enhancer function experiment description: |
Importantly, by CRISPR/Cas9-mediated genomic editing, we uncover functional hierarchy of constituent enhancers within the SLC25A37 super-enhancer. Despite indistinguishable chromatin features, we reveal through genomic editing the functional diversity of several GATA switch enhancers in which enhancers with opposing functions cooperate to coordinate transcription. |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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