About Enhancer
| Enhancer ID: | E_02_171 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr14:119710660-119714758   |
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| Biosample name: | G1ER Erythroid Cell | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 26766440 |
| Enhancer experiment: | CRISPR/Cas9,ChIP-seq | |
| Enhancer experiment description: | Specifically, we analyzed active enhancer-associated histone modifications H3K4me1 and H3K27ac by chromatin immunoprecipitation sequencing (ChIP-seq) and transcriptomic profiles in four distinct populations of human HSPCs or ProEs at fetal or adult stage (Figure 1B-E; Figure S1A).Identification of lineage or developmental stage-specific enhancers. Venn diagram shows the overlap between HSPC and ProE, or fetal and adult enhancers. The numbers of lost, shared, or gained enhancers in each comparison are shown. |
About Target gene
| Target gene : | Slc25a37(HT015,MFRN,MFRN1,MSC,MSCP,PRO1278,PRO1584,PRO2217) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP-seq,ChIP-qPCR | |
| Target gene experiment description: | Specifically, while deletion of E1 or E2 individually only modestly or slightly impairs Slc25a37 activation during differentiation, respectively, E3 deletion abolishes its activation resulting in a 15-fold decrease in expression (48h after β-estradiol treatment; Figure 3C). |
About TF
| TF name : | Gata1(ERYF1,GATA-1,GF-1,GF1,NF-E1,NFE1,XLANP,XLTDA,XLTT)Gata2(DCML,IMD21,MONOMAC,NFE1B) | |
| TF experiment: | ChIP-seq,CRISPR/Cas9 | |
| TF experiment description: | To further dissect the role of GATA switch in enhancer turnover, we compared GATA2 and GATA1 occupancy within A0 and A5 enhancers by ChIP-seq. Specifically, we enumerated the distribution of enhancers occupied by GATA2-Only, GATA1-Only, or GATA2-to-GATA1 switch.We then employed genomic editing to dissect the requirement of GATA switch enhancers within the paradigmatic Gata2 locus. |
About Function
| Enhancer function : | GATA2-to-GATA1 switch is prevalent at dynamic enhancers and drives erythroid enhancer commissioning. |
| Enhancer function experiment: | CRISPR/Cas9 |
| Enhancer function experiment description: |
Importantly, by CRISPR/Cas9-mediated genomic editing, we uncover functional hierarchy of constituent enhancers within the SLC25A37 super-enhancer. Despite indistinguishable chromatin features, we reveal through genomic editing the functional diversity of several GATA switch enhancers in which enhancers with opposing functions cooperate to coordinate transcription. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
