Enhancer ID: | E_02_159 | |
Enhancer symbol: | -- | |
Species: | Mouse | |
Position : | chr12:85466416-85469570 |
|
Biosample name: | Mouse Cortical Neurons | |
Experiment class : | Low+High throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 26595656 |
Enhancer experiment: | ChIP-seq,Luciferase Reporter Assay | |
Enhancer experiment description: | These enhancers, which we identified in primary cultures of mouse cortical neurons, appear to be active in the intact brain throughout development, as all of them were tightly aligned with the chroma_x0002_tin immunoprecipitation (ChIP)-seq peaks of the active enhancer marker, acetylated H3K27 (H3K27ac)(Supplementary Fig. 1). |
Target gene : | Fos(D12Rfj1,c-fos,cFos) | |
Strong evidence: | 3C | |
Less strong evidence: | -- | |
Target gene experiment description: | Characterizing individual c-fos Enhancers by chromosome-conformation capture (3C) analysis and an Enhancer reporter assay, we have corroborated the idea that eRNA is a reliable marker for identifying activated Enhancers. |
TF name : | Creb(Creb)srf(AW049942,AW240594)Mef2a(A430079H05Rik)Mef2d(C80750)Mef2c(5430401D19Rik,9930028G15Rik,AV011172,Mef2)Npas4(LE-PAS,Nxf) | |
TF experiment: | ChIP-seq | |
TF experiment description: | ChIP-_x0002_seq analysis has revealed that the c-fos promoter and enhancers were bound by different combinations of several activity-regulated TFs6 (Supplementary Fig. 1).Knockdown of CREB, MEF2A and NPAS4 significantly impaired c-fos induction by Cl-mediated membrane depolarization (Fig. 4a and Supplementary Fig. 4). Although knockdown of MEF2 family members MEF2D and MEF2C showed a slight decrease in c-fos expression, it was not significant and had a weaker impact than MEF2A knockdown. The decrease in c-fos expression was paralleled with little effect on eRNA transcription (Supplementary Fig. 5). |
Enhancer function : | These results demonstrate that the combinatorial activation of the c-fosenhancers enables the c-fos gene to be broadly responsive to various signaling pathways. |
Enhancer function experiment: | CRISPRi,RT-qPCR |
Enhancer function experiment description: |
We also evaluated the functionality of individual enhancers in their native context by targeted transcription silencing via CRISPR interference (CRISPRi). Using eRNA as a proxy for enhancer activity, we found that distinc_x0002_tive subsets of the c-fos enhancers are activated in the intact brain following chemically induced seizure or light stimulation. |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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