About Enhancer
| Enhancer ID: | E_02_144 | |
| Enhancer symbol: | Id2 Enhancer | |
| Species: | Mouse | |
| Position : | chr12:25090158-25090663   |
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| Biosample name: | HCT 116,SW480 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Colorectal Carcinoma | |
| DO: | DOID:0080199 | |
| Mesh: | D015179 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 26163528 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP-PCR,ChIP | |
| Enhancer experiment description: | Expression of Id2 was stimulated by Wnt signaling through the enhancer region of the Id2 promoter at the early stage of tumorigenesis in Apc+/Δ716 (ApcΔ716) mice. Genetic depletion of Id2 in ApcΔ716 mice caused ∼80% reduction in the number of ileal polyps, but had little effect on tumor size. |
About Target gene
| Target gene : | Id2(GIG8,ID2A,ID2H,bHLHb26) | |
| Strong evidence: | -- | |
| Less strong evidence: | Transgenic mice,RT-PCR,Western blot | |
| Target gene experiment description: | Expression of Id2 was stimulated by Wnt signaling through the enhancer region of the Id2 promoter at the early stage of tumorigenesis in Apc+/Δ716 (ApcΔ716) mice. Genetic depletion of Id2 in ApcΔ716 mice caused ∼80% reduction in the number of ileal polyps, but had little effect on tumor size. |
About TF
| TF name : | Tcf4(E2-2,FECD3,ITF-2,ITF2,PTHS,SEF-2,SEF2,SEF2-1,SEF2-1A,SEF2-1B,SEF2-1D,TCF-4,bHLHb19) | |
| TF experiment: | ChIP-PCR,Luciferase Reporter Assay | |
| TF experiment description: | To determine if mouse Id2 is also a target gene of Wnt signaling, we performed ChIP-PCR analysis of the binding of Tcf4 to the promoter region of the Id2 gene.Sequencing analysis revealed ten putative Tcf/Lef-binding consensus sequences in the Id2 promoter region spanning from ?4000 to ?1. As expected, Tcf4 bound to the DNA region spanning from ?3300 to ?3146 of the Id2 distal promoter.To test this Tcf4 binding region for enhancer activity, we conducted luciferase reporter assays using the constructs .The putative Id2-enhancer fragment significantly activated Wnt-signaling dependent transcription from the CMV minimal promoter in two colorectal cancer cell lines. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
