About Enhancer
| Enhancer ID: | E_02_134 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr8:106610150-106612150   |
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| Biosample name: | Non-tumorigenic Mouse Mammary Gland Cells | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 25652130 |
| Enhancer experiment: | Luciferase Reporter Assay,3C | |
| Enhancer experiment description: | We reported previously that intron 2 of Cdh1 is essential for proper E-cad expression.In order to analyze this large DNA sequence(>43 kb) for putative enhancers we focused on evolutionary conserved sequences. |
About Target gene
| Target gene : | Cdh1(Arc-1,BCDS1,CD324,CDHE,ECAD,LCAM,UVO) | |
| Strong evidence: | 3C | |
| Less strong evidence: | ChIP,qPCR | |
| Target gene experiment description: | We show that Cdh1 activity during MET is governed by two Enhancers at +7.8 kb and at +11.5 kb within intron 2 that are activated by binding of Grhl3 and Hnf4α,respectively. |
About TF
| TF name : | Grhl3(SOM,TFCP2L4,VWS2)Hnf4a(HNF-4,Hnf4lpha,MODY1,Nr2a1,TCF-14,Tcf14,Hnf4a) | |
| TF experiment: | Luciferase Reporter Assay,ChIP | |
| TF experiment description: | Using luciferase reporter assays,we first tested whether Hnf4α could activate Cl.5 in NMuMG cells.We found that indeed Hnf4α was able to exert a robust enhancing effect on the E-cad Cl.5 reporter construct.Two different mutations in the putative Hnf4α binding site decreased the basal activity of this construct and inhibited its Hnf4α dependent activation. |
About Function
| Enhancer function : | Enhancer cooperativity as a novel mechanism underlying the transcriptional regulation of E-cadherin during mesenchymal to epithelial transition. |
| Enhancer function experiment: | ChIP |
| Enhancer function experiment description: |
Enhancer cooperativity as a novel mechanism underlying the transcriptional regulation of E-cadherin during mesenchymal to epithelial transition. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
