About Enhancer
| Enhancer ID: | E_02_122 | |
| Enhancer symbol: | ECR1 Enhancer | |
| Species: | Mouse | |
| Position : | chr4:136140654-136141162   |
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| Biosample name: | Hep G2 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 25505291 |
| Enhancer experiment: | Luciferase Reporter Assay | |
| Enhancer experiment description: | Because enhancers were previously described for Id3 (32), we searched for evolutionally conserved regions that may function as enhancers and enable Smad2/3-dependent upregulation of Id3 in macrophages. Using ECR browser, we found two conserved regions, ECR1 located between -3177 and -2660 bp upstream of transcription start and ECR2 located between +4517 and 4662 bp downstream of the gene . ECR1 overlaps with the enhancer described by Shepherd et al. whereas ECR2 has not been described to date.Luciferase activity of pGL3-ID3prom ECR1 ECR2 plasmid cotransfected with plasmids containing BMP-specific Smad1 and Smad5. |
About Target gene
| Target gene : | Id3(HEIR-1,bHLHb25) | |
| Strong evidence: | -- | |
| Less strong evidence: | siRNA,RT-PCR | |
| Target gene experiment description: | To assess the effect of chromatin structure on Id3 gene expression, we used one pan–-HDAC inhibitor (TSA) and two selective HDAC inhibitors: MS-275, an inhibitor of HDAC1, and Apicidin, an inhibitor of HDAC2 and HDAC4. |
About TF
| TF name : | Tgfb1(TGF-beta1,TGFbeta1,Tgfb,Tgfb-1) | |
| TF experiment: | Western blot | |
| TF experiment description: | Western blot analysis of Smad phosphorylation upon stimulation of primary Human monocytederived macrophages with TGF-b1,BMPs,and their combinations. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
