About Enhancer
| Enhancer ID: | E_02_110 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr11:102006458-102008458   |
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| Biosample name: | Osteocyte Cell Lines | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 25271055 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP | |
| Enhancer experiment description: | Three lines of evidence support a direct role for HDAC5 in regulating MEF2C activity at the +45 kB SOST enhancer over the course of osteocyte differentiation. First, HDAC5 shRNA causes increased activity of this element, but not of the proximal SOST promoter, in luciferase assays. MEF2C shRNA reduces the activity of this enhancer. Second, HDAC5 overexpression dose-dependently inhibits the activity of a MEF2-driven reporter from the desmin and the SOST’9’ (+45kB) enhancer. Third, endogenous HDAC5 association with this region in control (but not HDAC5 shRNA) Ocy454 cells can be detected by ChIP. |
About Target gene
| Target gene : | Sost(5430411E23Rik) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP,qRT-PCR,Western blot | |
| Target gene experiment description: | Using Chromatin immunoprecipitation,we have mapped endogenous MEF2C binding in the SOST gene to a distal intergenic Enhancer 45 downstream from the transcription start site. |
About TF
| TF name : | Mef2c(5430401D19Rik,9930028G15Rik,AV011172,Mef2) | |
| TF experiment: | ChIP,PCR | |
| TF experiment description: | We performed Chromatin immunoprecipitation (ChIP) to determine MEF2C occupancy in Ocy454 cell cultured at 37°C for 14 days (a time point in which SOST expression is High throughput). |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
