About Enhancer
| Enhancer ID: | E_02_103 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr6:122700483-122702483   |
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| Biosample name: | Embryonic Stem Cell | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 25223790 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP,ChIP-qPCR | |
| Enhancer experiment description: | We also designed gRNA constructs and TALE-As to target the Nanog 5 kb upstream enhancer. Similar to the Oct4 locus, dCas9-As could bind their targeted regions and effectively activate the luciferase reporter carrying the 5 kb upstream enhancer |
About Target gene
| Target gene : | Nanog(NANOG) | |
| Strong evidence: | CRISPR/Cas9 | |
| Less strong evidence: | qRT-PCR,Luciferase Reporter Assay | |
| Target gene experiment description: | For Nanog luciferase assay reporter, the ～1.0 kb DNA frag_x0002_ment of the Nanog 5 kb Enhancer (?5145 to ?4154) was cloned into a mini promoter luciferase vector. |
About TF
| TF name : | Klf4(EZF,GKLF)Oct4Nanog(NANOG)Sox2(ANOP3,MCOPS3) | |
| TF experiment: | ChIP-seq | |
| TF experiment description: | To address this possibility, we reviewed the ChIP-seq information of several pluripotency transcription factors, including KLF4,OCT4,NANOG and SOX2 at the Nanog 5kb upstream Enhancer region(42) and found that the Site 2 (targeted by both TALE and dCas9) was surrounded by the predicted KLF4 and NANOG binding sites. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
