Enhancer ID: | E_02_074 | |
Enhancer symbol: | -- | |
Species: | Mouse | |
Position : | chr6:115409226-115436116 |
|
Biosample name: | Embryonic Stem Cell | |
Experiment class : | Low+High throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 24368734 |
Enhancer experiment: | ChIP-seq,qPCR,RNA-seq | |
Enhancer experiment description: | To characterize the genomic features of MLL4 binding regions during differentiation, we performed ChIP-Seq of H3K4me1/2/3, H3K27ac and Pol II during adipogenesis. Then, we used histone marks to define four types of gene regulatory elements: active enhancer, silent enhancer, active promoter, and silent promoter. Average profile plots revealed that at day 2 of adipogenesis, MLL4 and adipogenic TFs C/EBPα, C/EBPβ and PPARγ were enriched on active enhancers. |
Target gene : | Pparg(Nr1c3,PPAR-gamma,PPAR-gamma2,PPARgamma,PPARgamma2) | |
Strong evidence: | -- | |
Less strong evidence: | qPCR | |
Target gene experiment description: | Importantly, deletion of Mll4 markedly decreased H3K4me1 and H3K27ac on over 90% of the 591 recovered C/EBPβ+ MLL4+ adipogenic Enhancers (Figure 8C–D). MLL4-dependent de novo H3K4me1 and H3K27ac were also observed on the C/EBPβ+ adipogenic Enhancers located on Pparg gene locus. |
TF name : | Ppargc1a(A830037N07Rik,Gm11133,PGC-1,PPARGC-1-alpha,Pgc-1alpha,Pgc1,Pgco1,Ppargc1)Cebpa(C/EBP-alpha,CEBP)Cebpb(C/EBP-beta,IL6DBP,NF-IL6,TCF5) | |
TF experiment: | ChIP-seq | |
TF experiment description: | To experimentally validate the predicted motifs, we performed ChIP-Seq of C/EBPα, C/EBPβ and PPARγ at day 2 of adipogenesis and of MyoD in myocytes. By comparing the genomic localizations of MLL4 with those of C/EBPα, C/EBPβ and PPARγ, we found that consistent with the motif analysis, ~64% of MLL4 binding regions overlapped with those of C/EBPα/β or PPARγ at day 2 of adipogenesis. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
-- |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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