About Enhancer

Enhancer ID: E_02_074
Enhancer symbol: --
Species: Mouse
Position : chr6:115409226-115436116
Biosample name: Embryonic Stem Cell
Experiment class : Low+High throughput
Enhancer type: Enhancer
Disease: --
DO: --
Mesh: --
Distance from TSS: >2KB
Pubmed ID:  24368734
Enhancer experiment: ChIP-seq,qPCR,RNA-seq
Enhancer experiment description: To characterize the genomic features of MLL4 binding regions during differentiation, we performed ChIP-Seq of H3K4me1/2/3, H3K27ac and Pol II during adipogenesis. Then, we used histone marks to define four types of gene regulatory elements: active enhancer, silent enhancer, active promoter, and silent promoter. Average profile plots revealed that at day 2 of adipogenesis, MLL4 and adipogenic TFs C/EBPα, C/EBPβ and PPARγ were enriched on active enhancers.

About Target gene

Target gene : Pparg(Nr1c3,PPAR-gamma,PPAR-gamma2,PPARgamma,PPARgamma2)
Strong evidence: --
Less strong evidence: qPCR
Target gene experiment description: Importantly, deletion of Mll4 markedly decreased H3K4me1 and H3K27ac on over 90% of the 591 recovered C/EBPβ+ MLL4+ adipogenic Enhancers (Figure 8C–D). MLL4-dependent de novo H3K4me1 and H3K27ac were also observed on the C/EBPβ+ adipogenic Enhancers located on Pparg gene locus.

About TF

TF name : Ppargc1a(A830037N07Rik,Gm11133,PGC-1,PPARGC-1-alpha,Pgc-1alpha,Pgc1,Pgco1,Ppargc1)Cebpa(C/EBP-alpha,CEBP)Cebpb(C/EBP-beta,IL6DBP,NF-IL6,TCF5)
TF experiment: ChIP-seq
TF experiment description: To experimentally validate the predicted motifs, we performed ChIP-Seq of C/EBPα, C/EBPβ and PPARγ at day 2 of adipogenesis and of MyoD in myocytes. By comparing the genomic localizations of MLL4 with those of C/EBPα, C/EBPβ and PPARγ, we found that consistent with the motif analysis, ~64% of MLL4 binding regions overlapped with those of C/EBPα/β or PPARγ at day 2 of adipogenesis.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:
--

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs