About Enhancer

Enhancer ID: E_02_070
Enhancer symbol: --
Species: Mouse
Position : chr2:119321593-119322529
Biosample name: 293T,Progenitor Cell
Experiment class : Low throughput
Enhancer type: Enhancer
Disease: --
DO: --
Mesh: --
Distance from TSS: >2KB
Pubmed ID:  24257627
Enhancer experiment: ChIP,PCR,EMSA
Enhancer experiment description: In addition, we carried out chromatin immunoprecipitation (ChIP) assays to show that both Ascl1 and Neurog1 were able to occupy in vivo the critical E-box region of the CR1 Enhancer in cell culture and mouse embryonic neural tubes, whereas no enrichment was shown for control DNA in Dll4 3′ UTR.

About Target gene

Target gene : Dll4(Delta4)
Strong evidence: --
Less strong evidence: EMSA
Target gene experiment description: We performed an electrophoretic mobility shift assay (EMSA) to test whether Ascl1 and Neurog proteins were able to directly bind to the minimal enhancer region using an oligo probe containing the critical E-box (Probe 2) and a control upstream probe that lacks an E-box (Probe 1)

About TF

TF name : Foxn4(Foxn4)Ascl1(AI225900,ASH1,Mash1,bHLHa46)
TF experiment: EMSA,ChIP
TF experiment description: We performed an electrophoretic mobility shift assay (EMSA) to test whether Ascl1 and Neurog proteins were able to directly bind to the minimal Enhancer region using an oligo probe containing the critical E-box (Probe 2) and a control upstream probe that lacks an E-box.

About Function

Enhancer function : Foxn4, Ascl1 and Neurog1 were able to activate gene expression through an evolutionarily conserved Dll4 Enhancer CR1 found to be active in the retina.
Enhancer function experiment: ChIP,EMSA
Enhancer function
experiment description:
In addition, we carried out chromatin immunoprecipitation (ChIP) assays to show that both Ascl1 and Neurog1 were able to occupy in vivo the critical E-box region of the CR1 Enhancer in cell culture and mouse embryonic neural tubes, whereas no enrichment was shown for control DNA in Dll4 3′ UTR.Thus, Ascl1 and Neurog factors may bind to the same E-box in the Dll4 Enhancer but have differential effects on Dll4 gene expression.

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs