About Enhancer

Enhancer ID: E_02_066
Enhancer symbol: --
Species: Mouse
Position : chr5:28495262-28497421
Biosample name: Colon Adenocarcinoma Cell Lines
Experiment class : Low+High throughput
Enhancer type: Enhancer
Disease: --
DO: --
Mesh: --
Distance from TSS: >2KB
Pubmed ID:  24157522
Enhancer experiment: RT-PCR,ChIP-seq,Transient Transfection Assay,Luciferase Reporter Assay,3C,DNaseI-seq
Enhancer experiment description: The ChIP-seq data of the ENCODE project show that H3K4me1, but not H3K4me3, marks all four DNaseI HS sites in the adult lung.To examine whether each of the DNase I HS sites is responsible for upregulating the expression of Shh, we employed a transgenic reporter assay using BAC clones (Figs. 1A and 3A).

About Target gene

Target gene : Shh(HHG1,HLP3,HPE3,MCOPCB5,SMMCI,ShhNC,TPT,TPTPS)
Strong evidence: 3C
Less strong evidence: Luciferase Reporter Assay
Target gene experiment description: To determine whether SLGE physically interacts with the Shh promoter in association with transcriptional activity, we conducted a 3C assay, which has been used to measure associations between two genomic regions. Assessment of the functional properties of SLGE by 3C and luciferase assays.

About TF

TF name : Rbfox3(Fox-3,Hrnbp3,NeuN,Neuna60)Socs1(Cish1,Cish7,JAB,SOCS-1,SSI-1)
TF experiment: DNaseI-seq
TF experiment description: Putative binding sites for Fox transcription factors and STAT are found in the SLGE.In human cancer cell lines, including Caco-2, putative Fox- and STAT-binding sites are found in some of the DNase I HS sites around the SHH CDS.

About Function

Enhancer function : Shh expression in the endodermal epithelial lining, which is enabled by the coordinated actions of the Enhancers, is necessary for proper endodermal development.
Enhancer function experiment: RT-PCR,Luciferase Reporter Assay,3C
Enhancer function
experiment description:
We identified a novel, less-conserved cis-regulatory element for Shh expres_x005f_x0002_sion in the esophageal epithelium, as well as in the lung and intestinal epithelium, in a region 100 Kb upstream of the Shh CDS. The new element compensates for the Enhancer activi_x0002_ties of the three previously identified Enhancers, and the combined activities of all four Enhancers permit the seamless Shh expression in the epithelial linings of the whole endoder_x0002_mal tissues in the mouse embryo.

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs