Enhancer ID: | E_02_060 | |
Enhancer symbol: | CE Enhancer | |
Species: | Mouse | |
Position : | chr7:46352720-46353957 |
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Biosample name: | C2C12 | |
Experiment class : | Low+High throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 23993744 |
Enhancer experiment: | siRNA,ChIP-qPCR,qRT-PCR,ChIP-seq,DNaseI-seq | |
Enhancer experiment description: | To this end, we devised a screening approach where ten different small interfering RNA duplexes (siRNA) were designed to target various regions upstream of MYOD1 with proximity to MyoD+/MyoG+/H3K4me1+ peaks (Figure 3A, #1–10). As controls, siRNAs were designed to target four eRNA+ regions on other chromosomes (Figure S3) and another against green fluorescent protein (GFPi). Transfected C2C12 cells were placed in differentiation media (DM) for 4–6hrs and harvested thereafter for analysis. |
Target gene : | Myod(AI503393,MYF3,MyoD,Myod-1,bHLHc1) | |
Strong evidence: | -- | |
Less strong evidence: | ChIP-seq,RNA-seq | |
Target gene experiment description: | Within this region, the concerted activity of two DNA Enhancer elements, CE and DRR, specifies the level of MyoD expression in the myogenic lineage.In this context, we surveyed for the enhancer signature (i.e. H3K4me1) within ~1–2kb of MyoG+ sites. We obtained and analyzed ChIP-Seq data for H3K4me1 (Table S1) and observed the majority (~80%) of the extragenic MyoG+ peaks within H3K4me1+ domains (Figure 1B–D).To ascertain whether these regions were transcriptionally active, we performed paired-end RNA-Seq (PE-Seq)from ribosome-depleted RNA fraction from MT (76bp/end, see Table S1 for number of reads). |
TF name : | Mef2a(A430079H05Rik)Foxo1(FKH1,FKHRA,FOXO1)Myod1(AI503393,MYF3,MyoD,Myod-1,bHLHc1)Myog | |
TF experiment: | qRT-PCR,ChIP-seq | |
TF experiment description: | Similar to other nuclear eRNAs examined (FigureS1G–I), DRRRNA levels were significantly reduced following MyoDi and to a lesser extent by MyoGi (Figure 2D), suggesting that its transcriptional regulation is under the concerted control of these transcription factors. |
Enhancer function : | The eRNAs regulate genomic access of the transcriptional complex to defined regulatory regions. |
Enhancer function experiment: | ChIP |
Enhancer function experiment description: |
The above findings prompted us to evaluate whether RNA-assisted PolII recruitment could also explain the effect of DRRRNA on MyoG expression (Figure 4A,B). Indeed, ChIP experiments revealed that DRRi resulted in the reduction of PolII at MYOG, but not at MYOD1. |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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