About Enhancer
| Enhancer ID: | E_02_056 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr17:26829229-26831311   |
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| Biosample name: | Cardiac Progenitor Cell | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 23307821 |
| Enhancer experiment: | ChIP,PCR,Luciferase Reporter Assay,EMSA | |
| Enhancer experiment description: | To examine in detail the potential binding of YY1 to Nkx2.5 enhancer, we performed an electromobility shift assay (EMSA) using the 2.1 kb cardiac enhancer located within -9435 and -7353 bases upstream from the murine NKX2.5 transcriptional start site.Binding of site #1 to YY1 was further confirmed using a DNA supershift assay (Figure 2C). To corroborate these in vitro data with their binding to the Nkx2.5 cardiac enhancer in vivo , we performed chromatin immunoprecipitation (ChIP) and PCR analysis using anti-YY1 antibodies on day 6 differentiated NK ESCs (Figure 2D), FACS-purified eGFP+ CPCs and eGFP- cells from day 6 differentiated NK ESCs (Figure 2E), and from embryonic day 9.5 hearts and body. |
About Target gene
| Target gene : | Nkx2-5(CHNG5,CSX,CSX1,HLHS2,NKX2.5,NKX2E,NKX4-1,VSD3) | |
| Strong evidence: | -- | |
| Less strong evidence: | EMSA,ChIP,PCR | |
| Target gene experiment description: | To examine in detail the potential binding of YY1 to Nkx2.5 enhancer, we performed an electromobility shift assay (EMSA) using the 2.1 kb cardiac enhancer located within -9435 and -7353 bases upstream from the murine NKX2.5 transcriptional start site.Binding of site #1 to YY1 was further confirmed using a DNA supershift assay (Figure 2C). To corroborate these in vitro data with their binding to the Nkx2.5 cardiac enhancer in vivo , we performed chromatin immunoprecipitation (ChIP) and PCR analysis using anti-YY1 antibodies on day 6 differentiated NK ESCs (Figure 2D), FACS-purified eGFP+ CPCs and eGFP- cells from day 6 differentiated NK ESCs (Figure 2E), and from embryonic day 9.5 hearts and body. |
About TF
| TF name : | Yy1(DELTA,GADEVS,INO80S,NF-E1,UCRBP,YIN-YANG-1) | |
| TF experiment: | ChIP,Luciferase Reporter Assay,EMSA | |
| TF experiment description: | YY1 regulates Nkx2.5 expression via a 2.1 kb cardiac-specific enhancer as demonstrated by in vitro luciferase-based assays and in vivo chromatin immunoprecipitation (ChIP) and genome-wide sequencing analysis.To examine in detail the potential binding of YY1 to Nkx2.5 enhancer, we performed an electromobility shift assay (EMSA) using the 2.1 kb cardiac enhancer located within -9435 and -7353 bases upstream from the murine NKX2.5 transcriptional start site. |
About Function
| Enhancer function : | YY1 regulates Nkx2.5 expression via a 2.1-kb cardiac-specific enhancer |
| Enhancer function experiment: | EMSA,ChIP,PCR |
| Enhancer function experiment description: |
To examine in detail the potential binding of YY1 to Nkx2.5 Enhancer, we performed an electromobility shift assay (EMSA) using the 2.1 kb cardiac Enhancer located within -9435 and -7353 bases upstream from the murine NKX2.5 transcriptional start site. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
