Enhancer ID: | E_02_053 | |
Enhancer symbol: | GnRH-E3 Enhancer | |
Species: | Mouse | |
Position : | chr14:67740982-67741286 |
|
Biosample name: | GT1-7 | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | Polycystic Ovarian Syndrome | |
DO: | DOID:11612 | |
Mesh: | D011085 | |
Distance from TSS: | >2KB | |
Pubmed ID: | 22877652 |
Enhancer experiment: | ChIP,PCR,Luciferase Reporter Assay,EMSA | |
Enhancer experiment description: | To determine whether AR interacts with endogenous chromatin in the GnRH-E1 region in GT1-7 cells, ChIP assays were performed. GT1-7 cells were transiently transfected with luciferase reporter constructs containing the GnRH-P (P), GnRH-E1 (E1), and/or GnRH-E2 (E2) regions, and/or the RSV promoter (RSVp) or enhancer (RSVe), as indicated, along with the AR expression vector. |
Target gene : | Gnrh1(Gnrh,Gnrh2,LHRH,Lhrh1,Lnrh,hpg) | |
Strong evidence: | -- | |
Less strong evidence: | Luciferase Reporter Assay | |
Target gene experiment description: | AR associates with the enhancer 1 region of the GnRH regulatory region and represses transcription.GT1-7 cells were transiently transfected with luciferase reporter constructs containing the GnRH-P (P), GnRH-E1 (E1),and/or GnRH-E2 (E2) regions, and/or the RSV promoter (RSVp) or enhancer (RSVe), as indicated, along with the AR expression vector. |
TF name : | Oct1Pbx(Pbx)Nkx2-1(BCH,BHC,NK-2,NKX2.1,NKX2A,NMTC1,T/EBP,TEBP,TITF1,TTF-1,TTF1) | |
TF experiment: | EMSA,PCR | |
TF experiment description: | AR bound to the -1796/-1791 sequence in electrophoretic mobility shift assays, indicating a direct interaction with DNA or other transcription factors in this region. |
Enhancer function : | AR represses GnRH transcription via the major Enhancer (GnRH-E1). |
Enhancer function experiment: | ChIP |
Enhancer function experiment description: |
For the rest of this study, we chose to evaluate the mechanism of repression by AR using GnRH-E1 upstream of RSVp to avoid com_x005f_x0002_pensation by the androgen-responsive GnRH promoter.To determine whether AR interacts with endogenous chromatin in the GnRH-E1 region in GT1-7 cells,ChIP assays were performed. |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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