About Enhancer

Enhancer ID: E_02_053
Enhancer symbol: GnRH-E3 Enhancer
Species: Mouse
Position : chr14:67740982-67741286
Biosample name: GT1-7
Experiment class : Low throughput
Enhancer type: Enhancer
Disease: Polycystic Ovarian Syndrome
DO: DOID:11612
Mesh: D011085
Distance from TSS: >2KB
Pubmed ID:  22877652
Enhancer experiment: ChIP,PCR,Luciferase Reporter Assay,EMSA
Enhancer experiment description: To determine whether AR interacts with endogenous chromatin in the GnRH-E1 region in GT1-7 cells, ChIP assays were performed. GT1-7 cells were transiently transfected with luciferase reporter constructs containing the GnRH-P (P), GnRH-E1 (E1), and/or GnRH-E2 (E2) regions, and/or the RSV promoter (RSVp) or enhancer (RSVe), as indicated, along with the AR expression vector.

About Target gene

Target gene : Gnrh1(Gnrh,Gnrh2,LHRH,Lhrh1,Lnrh,hpg)
Strong evidence: --
Less strong evidence: Luciferase Reporter Assay
Target gene experiment description: AR associates with the enhancer 1 region of the GnRH regulatory region and represses transcription.GT1-7 cells were transiently transfected with luciferase reporter constructs containing the GnRH-P (P), GnRH-E1 (E1),and/or GnRH-E2 (E2) regions, and/or the RSV promoter (RSVp) or enhancer (RSVe), as indicated, along with the AR expression vector.

About TF

TF name : Oct1Pbx(Pbx)Nkx2-1(BCH,BHC,NK-2,NKX2.1,NKX2A,NMTC1,T/EBP,TEBP,TITF1,TTF-1,TTF1)
TF experiment: EMSA,PCR
TF experiment description: AR bound to the -1796/-1791 sequence in electrophoretic mobility shift assays, indicating a direct interaction with DNA or other transcription factors in this region.

About Function

Enhancer function : AR represses GnRH transcription via the major Enhancer (GnRH-E1).
Enhancer function experiment: ChIP
Enhancer function
experiment description:
For the rest of this study, we chose to evaluate the mechanism of repression by AR using GnRH-E1 upstream of RSVp to avoid com_x005f_x0002_pensation by the androgen-responsive GnRH promoter.To determine whether AR interacts with endogenous chromatin in the GnRH-E1 region in GT1-7 cells,ChIP assays were performed.

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs