About Enhancer
| Enhancer ID: | E_02_045 | |
| Enhancer symbol: | Scl +19 Enhancer | |
| Species: | Mouse | |
| Position : | chr4:115074426-115076426   |
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| Biosample name: | Hematopoietic Stem Cell,Progenitor Cell | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Acute Leukemia | |
| DO: | DOID:12603 | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 22401818 |
| Enhancer experiment: | RT-PCR,qPCR,ChIP | |
| Enhancer experiment description: | Real-time semi-quantitative PCR performed with primers specific for the Scl gene [16] showed that expression levels of Scl in erythroid (Ter119 þ ), myeloid (Mac-1 þ ), megakaryocytic (CD41 þ ), and T-cell (CD4 þ ) lineages were similar for WTand Scl D19/D19 mice (Fig. 1D). To determine whether deletion of the Scl +19 enhancer affects embryonic/fetal hematopoiesis, we quantified the number of progenitors in the fetal liver using methylcellulose-based colony assay. |
About Target gene
| Target gene : | Tal1(SCL,TCL5,bHLHa17,tal-1) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP | |
| Target gene experiment description: | To identify possible redundancy between the þ19 enhancer and other Scl regulatory elements, we performed chromatin immunoprecipitation (ChIP) assays on E14.5 fetal liver cells with markers for repressive (H3K9me3 and H3K9me2) and active (H3K4me3 and H3K9Ac) chromatin. |
About TF
| TF name : | Spi1(OF,PU.1,SFPI1,SPI-1,SPI-A)Erg(erg-3,p55) | |
| TF experiment: | ChIP-seq | |
| TF experiment description: | Using whole-genome transcription factor binding mapping by ChIP-Seq,we have recently identified other important hematopoietic regulators that also bind to the Scl t19 Enhancer,including the Ets factors Spi1 and Erg. |
About Function
| Enhancer function : | The Scl +19 Enhancer plays a key role in the development of hematopoietic stem/progen_x005f_x0002_itor cells,but is not necessary for mature hematopoietic lineages. |
| Enhancer function experiment: | ChIP |
| Enhancer function experiment description: |
Interestingly,both active euchromatin marks are reduced in SclD19/D19 fetal liver cells,indicating that the Scl t19 element is required for enhanced activity of the Scl promoter. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
