About Enhancer
| Enhancer ID: | E_02_027 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr14:55518040-55518674   |
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| Biosample name: | HEK-293 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | <2KB | |
| Pubmed ID: | 21673114 |
| Enhancer experiment: | Transfection,EMSA,PCR | |
| Enhancer experiment description: | To refine the cis-regulatory elements involved in controlling Nrl expression further, we tested the sequences within the three conserved clusters by in vivo transfection. |
About Target gene
| Target gene : | Nrl(D14H14S46E) | |
| Strong evidence: | -- | |
| Less strong evidence: | EMSA | |
| Target gene experiment description: | In silico analysis using the TRANSFAC database (46) revealed a number of conserved putative transcription factor binding sites within B2(transcriptionally active sequence in clusterB)and clusterA(Fig.3,A and B, respectively). To identify the involvement of specific transcription factors, we performed EMSA using several different oligonucleotides spanning the conserved sequence elements(Fig. 4). |
About TF
| TF name : | Rorb(Nr1f2,RZR-beta,RZRBeta,hstp,Rorb)Crx(Crx1)NrlNr2e3 | |
| TF experiment: | EMSA | |
| TF experiment description: | Electrophoretic mobility shift assays using mouse retinal nuclear extracts, in combination with specific antibodies,demonstrate the binding of retinoid-related orphan nuclear receptor(RORβ),cone rod homeobox,orthodenticle homolog 2,and cyclic AMP response element-binding protein to predicted consensus elements within clusters A and B.Our studies demonstrate Nrl as a direct transcriptional target of RORβ and suggest that combinatorial action of multiple regulatory factors modulates the expression of Nrl in developing and mature retina. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
