Enhancer ID: | E_02_006 | |
Enhancer symbol: | MHB Enhancer | |
Species: | Mouse | |
Position : | chr4:44520195-44520234 |
|
Biosample name: | Lymphopoiesis B Cell | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 19345119 |
Enhancer experiment: | Transfection,ChIP,EMSA | |
Enhancer experiment description: | By using transgenic analysis, we have previously identified a 435 bp enhancer (located 5.6 kb upstream of exon 1A), which is sufficient to drive reporter gene expression in the developing MHB region.To confirm these footprinting data, we performed ChIP analysis with unstimulated cells of the mature B cell line WEHI-279 (Figure 7C; Figure S14A) and with in vitro cultured pro-B cells (Figure S14B) to screen all HS sites of the Pax5 locus for binding of PU.1, IRF4, IRF8, NF-kB, and Myc. Electrophoretic mobility shift assays(EMSAs)with an oligonucleotide probe containing the EBF1 recognition sequence of HS-7 revealed efficient protein binding to this site with a pro-B cell nuclear extract. |
Target gene : | Pax5(BSAP,EBB-1,KLP,Pax-5) | |
Strong evidence: | -- | |
Less strong evidence: | Transgenic mice | |
Target gene experiment description: | A DNA fragment containing the entire Pax5 promoter region from the MHB enhancer (at position -6,074) to exon 2 (at position +13,368) was fused in-frame in exon 2 to a Gfp reporter gene to generate transgene PromGFP.GFP expression was determined by flow cytometry in different B cell types of a PromGFP transgenic mouse. PromGFP transgenic mice failed to express GFP in pro-B and mature B cells, but gave rise to low GFP expression in pre-B and immature B cells(Figure 4A) similar to the BAC1-DIn5 transgene . |
TF name : | Spi1(OF,PU.1,SFPI1,SPI-1,SPI-A)Irf4(AI385587,IRF-4,LSIRF,NF-EM5,Spip)Irf8(H-ICSBP,ICSBP,ICSBP1,IMD32A,IMD32B,IRF-8)Nfkb1(CVID12,EBP-1,KBF1,NF-kB,NF-kB1,NF-kappa-B1,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50) | |
TF experiment: | ChIP,DMS Footprinting Assay | |
TF experiment description: | PU.1, IRF4, IRF8, and NF-kB Control the Activity of the Pax5 Enhancer.To confirm these footprinting data, we performed ChIP analysis with unstimulated cells of the mature B cell line WEHI-279 and with in vitro cultured pro-B cells to screen all HS sites of the Pax5 locus for binding of PU.1, IRF4, IRF8, NF-kB, and Myc. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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