About Enhancer
| Enhancer ID: | E_02_003 | |
| Enhancer symbol: | β-cell specific Enhancer | |
| Species: | Mouse | |
| Position : | chr3:51508734-51512187   |
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| Biosample name: | βTC-3,INS-1,αTC1.6,NIH3T3 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Insulin Deficiency | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 18984737 |
| Enhancer experiment: | Luciferase Reporter Assay | |
| Enhancer experiment description: | To directly test whether this region of the Setd7 gene might be regulated in β-cells, a DNA fragment containing this conserved region was placed upstream of the prolactin minimal promoter driving luciferase and used in reporter gene analysis studies.β-cell lines displayed enhancement of luciferase activity with a DNA fragment containing _x0003_6,584 bp upstream of the transcriptional start site. This enhancement was significantly attenuated when a fragment containing _x0003_3,131 bp from the transcriptional start site was used, suggesting the potential for a β-cell specific enhancer located between _x0003_3,131 and _x0003_6,584 bp. |
About Target gene
| Target gene : | Setd7(KMT7,SET7,SET7/9,SET9) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay | |
| Target gene experiment description: | β-cell lines displayed enhancement of luciferase activity with a DNA fragment containing _x0003_6,584 bp upstream of the transcriptional start site. This enhancement was significantly attenuated when a fragment containing _x0003_3,131 bp from the transcriptional start site was used, suggesting the potential for a β-cell specific enhancer located between _x0003_3,131 and _x0003_6,584 bp. |
About TF
| TF name : | Pdx1(GSF,IDX-1,IPF1,IUF1,MODY4,PAGEN1,PDX-1,STF-1) | |
| TF experiment: | RT-PCR,ChIP,Luciferase Reporter Assay | |
| TF experiment description: | To directly test whether this region of the Setd7 gene might be regulated in β-cells, a DNA fragment containing this conserved region was placed upstream of the prolactin minimal promoter driving luciferase and used in reporter gene analysis studies. As shown in Fig. 2B, this fragment displayed relative enhancement of luciferase activity in _x0001_TC3 and INS-1,β-cell lines,but not in NIH3T3 cells. Upon cotransfection with a plasmid encoding Pdx1, this fragment displayed an _x0008_25-fold enhancement in the β-cell lines INS-1 and_x0001_TC3. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
