About Enhancer
| Enhancer ID: | E_02_001 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr10:52388022-52388224   |
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| Biosample name: | P19 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | <2KB | |
| Pubmed ID: | 25209250 |
| Enhancer experiment: | Luciferase Reporter Assay | |
| Enhancer experiment description: | Luciferase constructs containing the Nepn TSS and upstream promoter regions were assayed in P19 cells treated with DMSO or RA (0.1 μM). The length of the upstream region for each construct is indicated in the graphic. Relative luciferase activity represents the fold increase observed in the presence of RA relative to DMSO. |
About Target gene
| Target gene : | Nepn(5730521E12Rik,Npn,periolin) | |
| Strong evidence: | CRISPR/Cas9 | |
| Less strong evidence: | Luciferase Reporter Assay,RT-PCR | |
| Target gene experiment description: | Luciferase assays indicated that promoter activity of the construct (2.3 kb) that contains both Sox17-binding motif and RARE was dramatically enhanced with ectopic expression of Sox17 in the presence of RA. |
About TF
| TF name : | Sox17(Sox17)Rara(Nr1b1,RAR,RARalpha1) | |
| TF experiment: | Luciferase Reporter Assay,EMSA | |
| TF experiment description: | The luciferase activity of these constructs was evaluated in P19 cells in the presence of ectopically expressed Sox17. A significant drop in Sox17-inducible activity occurred when the sequence between ?842?bp and ?640?bp upstream of the Nepn TSS was deleted, suggesting that an enhancer region that is dependent on Sox17 lies in this region.EMSA assay of nuclear protein binding to the proposed RARE from the Nepn promoter. Myc-tagge RARα was ectopically expressed in P19 cells treated with RA (0.1 μM) and nuclear extracts were incubated with 32P-labelled RARE from the Nepn promoter. Competition with a 100-fold excess of unlabeled wild-type or mutated RARE is indicated. Anti-Myc antibodies were included in the binding assay as indicated. Sindicates the RARα-DNA complex; SS indicates the super shifted complex in the presence of anti-Myc antibody. **P<0.01. Mut, mutated; NE, nuclear extracts; Wt, wild type. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
