About Enhancer

Enhancer ID: E_01_403
Enhancer symbol: --
Species: Human
Position : chr11:95817801-95818824
Biosample name: MCF10CA1h,MCF10CA1a
Experiment class : Low+High throughput
Enhancer type: Enhancer
Disease: Breast Cancer
DO: DOID:1612
Mesh: D001943
Distance from TSS: >2kb
Pubmed ID:  27207652
Enhancer experiment: ChIP,qPCR,Transfection,Western blot
Enhancer experiment description: The difference in DNA meth_x0002_ylation within MAML2 in RSV treated versus control cells was determined as 0.37 in MCF10CA1h and 0.1 in MCF10CA1a cells based on the array data. These elevated levels of methylation were identified in a fragment corresponding to the predic_x0002_tive enhancer within MAML2 gene body. It has been demonstrated that active enhancers are enriched with H3K27ac.We confirmed by ChIP assay that this histone mark is present at MAML2 in cancer cells and its occupancy is significantly reduced by RSV.These data strongly support the role of this region as a gene enhancer and the role of RSV in decreasing the activ_x0002_ity of this enhancer.

About Target gene

Target gene : MAML2(MAM-3,MAM2,MAM3,MLL-MAML2)
Strong evidence: --
Less strong evidence: ChIP,Transfection,Western blot
Target gene experiment description: MAML2, a coactivator of NOTCH targets, is methylated at the enhancer region and transcriptionally silenced in response to stilbenoids, possibly explaining the downregulation of NOTCH target genes.It has been demonstrated that active enhancers are enriched with H3K27ac.We confirmed by ChIP assay that this histone mark is present at MAML2 in cancer cells and its occupancy is significantly reduced by RSV.

About TF

TF name : POU2F1(OCT1,OTF1,oct-1B)
TF experiment: ChIP,qPCR
TF experiment description: Using ChIP, we confirmed OCT1 binding to MAML2 enhancer (Figure 6C, control cells). The binding dramati_x0002_cally decreases upon treatment of MCF10CA1a with 15 μM RSV.The lack of OCT1 binding may be directly linked to MAML2 transcriptional silencing. To test this hypothesis, we depleted OCT1 in MCF10CA1a cells using SmartPool siRNA mixture and four siRNA sequences separately, as confirmed by QPCR and western blot and tested the effect of OCT1 absence on MAML2 expression.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:
--

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs